Bicchi C, Appendino G, Cordero C, Rubiolo P, Ortelli D, Veuthey J L
Dipartimento di Scienza e Tecnologia del Farmaco, via Pietro Giuria 9, I-10125 Torino, Italy.
Phytochem Anal. 2001 Jul-Aug;12(4):255-62. doi: 10.1002/pca.592.
Caper spurge (Euphorbia lathyris L.) seed oil contains a series of diterpenoids known as Euphorbia factors, or L-factors, L1-L9. They are esters of several polyols (lathyrol, epoxylathyrol, hydroxylathyrol and ingenol) and account for about 3-5% of the oil. The percentage of ingenol-based L-factors is very low, less than 5% of the diterpenoid fraction, but some of them (factors L5 and L6) are responsible for the irritant and co-carcinogenic activities of the oil. This paper reports an HPLC-UV and HPLC-positive-ESI-MS analysis of the diterpenoid fraction of caper spurge seed oil before and after selective hydrolysis of ingenol-based L-factors. Separation of lathyrane polyols and esters, and ingenol and its esters was achieved using a chromatographic system consisting of a C18 stationary phase and acetonitrile: water as mobile phase. A new macrocyclic constituent, the deoxy Euphorbia factor L1, was identified in the oil.
续随子（大戟科续随子）种子油含有一系列被称为大戟因子或L因子（L1 - L9）的二萜类化合物。它们是几种多元醇（千金二萜醇、环氧千金二萜醇、羟基千金二萜醇和巨大戟醇）的酯，约占油的3 - 5%。基于巨大戟醇的L因子百分比非常低，不到二萜类部分的5%，但其中一些（因子L5和L6）是该油具有刺激性和协同致癌活性的原因。本文报道了对基于巨大戟醇的L因子选择性水解前后续随子种子油二萜类部分的高效液相色谱 - 紫外（HPLC - UV）和高效液相色谱 - 正电喷雾电离质谱（HPLC - positive - ESI - MS）分析。使用由C18固定相和乙腈：水作为流动相组成的色谱系统实现了千金二萜烷多元醇及其酯、巨大戟醇及其酯的分离。在该油中鉴定出一种新的大环成分，脱氧大戟因子L1。
