Decousser J W, Poirel L, Nordmann P
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, France.
Antimicrob Agents Chemother. 2001 Dec;45(12):3595-8. doi: 10.1128/AAC.45.12.3595-3598.2001.
A chromosomally located beta-lactamase gene, cloned and expressed in Escherichia coli from a reference strain of the enterobacterial species Kluyvera cryocrescens, encoded a clavulanic acid-inhibited Ambler class A enzyme, KLUC-1, with a pI value of 7.4. KLUC-1 shared 86% amino acid identity with a subgroup of plasmid-mediated CTX-M-type extended-spectrum beta-lactamases (CTX-M-1, -3, -10, -11, and -12), the most closely related enzymes, and 77% amino acid identity with KLUA-1 from Kluyvera ascorbata. The substrate profile of KLUC-1 corresponded to that of CTX-M-type enzymes.
一个位于染色体上的β-内酰胺酶基因，从肠杆菌科克吕沃尔氏菌（Kluyvera cryocrescens）的参考菌株中克隆并在大肠杆菌中表达，编码一种克拉维酸抑制的安布勒A类酶KLUC-1，其pI值为7.4。KLUC-1与质粒介导的CTX-M型超广谱β-内酰胺酶（CTX-M-1、-3、-10、-11和-12）的一个亚组（关系最密切的酶）共享86%的氨基酸同一性，与来自抗坏血酸克吕沃尔氏菌（Kluyvera ascorbata）的KLUA-1共享77%的氨基酸同一性。KLUC-1的底物谱与CTX-M型酶的底物谱一致。
