水生拉恩菌染色体编码的超广谱A类β-内酰胺酶的生化遗传学特性
Biochemical-genetic characterization of the chromosomally encoded extended-spectrum class A beta-lactamase from Rahnella aquatilis.
作者信息
Bellais S, Poirel L, Fortineau N, Decousser J W, Nordmann P
机构信息
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 78 rue de Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France.
出版信息
Antimicrob Agents Chemother. 2001 Oct;45(10):2965-8. doi: 10.1128/AAC.45.10.2965-2968.2001.
Abstract
From whole-cell DNA of a clinical isolate of the enterobacterial species Rahnella aquatilis, a beta-lactamase gene was cloned that encoded a chromosomally encoded Ambler class A enzyme, RAHN-1. RAHN-1, with a pI of 7.2, shares 76, 73, and 71% amino acid identity with the extended-spectrum beta-lactamase of chromosomal origin from Serratia fonticola and with the plasmid-mediated beta-lactamases CTX-M-2 and CTX-M-1, respectively. The hydrolysis spectrum of the clavulanic acid-inhibited RAHN-1 was expanded to cephalosporins such as cefuroxime, cefotaxime, and ceftriaxone, but not to ceftazidime. Its expression was not inducible.
摘要
从水生拉恩菌这一肠杆菌属临床分离株的全细胞DNA中，克隆出了一个β-内酰胺酶基因，该基因编码一种染色体编码的安布勒A类酶RAHN-1。RAHN-1的等电点为7.2，与粘质沙雷氏菌染色体来源的超广谱β-内酰胺酶分别有76%、73%和71%的氨基酸同一性，与质粒介导的β-内酰胺酶CTX-M-2和CTX-M-1也分别有76%、73%和71%的氨基酸同一性。克拉维酸抑制后的RAHN-1的水解谱扩展至头孢呋辛、头孢噻肟和头孢曲松等头孢菌素，但不包括头孢他啶。其表达不可诱导。
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