Kraemer P M, Petersen D F, Van Dilla M A
Science. 1971 Nov 12;174(4010):714-7. doi: 10.1126/science.174.4010.714.
Cellular DNA was measured by high-speed flow microfluorometry in mammalian diploid and heteroploid cell populations stained by the fluorescent Feulgen procedure. Heteroploid cells with elevated modal chromosome number showed the expected increase in modal DNA content. However, the variability of DNA content was the same in diploid and heteroploid cell populations despite the large variability of chromosome number in the latter populations. This suggests that heteroploidy may include defects in the chromosomal condensation and kinetochore development systems.
通过高速流动微荧光测定法,对经荧光福尔根染色法染色的哺乳动物二倍体细胞群体和异倍体细胞群体中的细胞DNA进行了测量。具有升高的众数染色体数的异倍体细胞显示出预期的众数DNA含量增加。然而,尽管后者群体中染色体数的变异性很大,但二倍体细胞群体和异倍体细胞群体中DNA含量的变异性是相同的。这表明异倍性可能包括染色体凝聚和动粒发育系统中的缺陷。