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异倍体中的DNA恒定性与肿瘤的干细胞系学说

DNA constancy in heteroploidy and the stem line theory of tumors.

作者信息

Kraemer P M, Petersen D F, Van Dilla M A

出版信息

Science. 1971 Nov 12;174(4010):714-7. doi: 10.1126/science.174.4010.714.

DOI:10.1126/science.174.4010.714
PMID:5001276
Abstract

Cellular DNA was measured by high-speed flow microfluorometry in mammalian diploid and heteroploid cell populations stained by the fluorescent Feulgen procedure. Heteroploid cells with elevated modal chromosome number showed the expected increase in modal DNA content. However, the variability of DNA content was the same in diploid and heteroploid cell populations despite the large variability of chromosome number in the latter populations. This suggests that heteroploidy may include defects in the chromosomal condensation and kinetochore development systems.

摘要

通过高速流动微荧光测定法,对经荧光福尔根染色法染色的哺乳动物二倍体细胞群体和异倍体细胞群体中的细胞DNA进行了测量。具有升高的众数染色体数的异倍体细胞显示出预期的众数DNA含量增加。然而,尽管后者群体中染色体数的变异性很大,但二倍体细胞群体和异倍体细胞群体中DNA含量的变异性是相同的。这表明异倍性可能包括染色体凝聚和动粒发育系统中的缺陷。

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DNA constancy in heteroploidy and the stem line theory of tumors.异倍体中的DNA恒定性与肿瘤的干细胞系学说
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引用本文的文献

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Cytogenetic instability of chromosomal nucleolar organizer regions (NORs) in cloned mouse L929 fibroblasts.克隆鼠 L929 成纤维细胞的染色体核仁组织者区域(NORs)的细胞遗传学不稳定性。
Chromosome Res. 2019 Mar;27(1-2):95-108. doi: 10.1007/s10577-018-9598-8. Epub 2019 Jan 3.
2
DNA ploidy and biologic aggressiveness of gastric adenocarcinoma in Chinese.
World J Surg. 1994 May-Jun;18(3):433-9; discussion 439-40. doi: 10.1007/BF00316831.
3
Cellular DNA content--a stable feature in epithelial ovarian cancer.细胞DNA含量——上皮性卵巢癌的一个稳定特征。
Br J Cancer. 1984 Feb;49(2):173-9. doi: 10.1038/bjc.1984.29.
4
Clinical and biological significance of aneuploidy in human tumours.人类肿瘤中非整倍体的临床及生物学意义。
J Clin Pathol. 1984 Sep;37(9):961-74. doi: 10.1136/jcp.37.9.961.
5
Gene dosage and the expression of electrophoretic patterns in heteroploid mouse cell lines.异倍体小鼠细胞系中的基因剂量与电泳图谱表达
Genetics. 1973 Jul;74(3):521-31. doi: 10.1093/genetics/74.3.521.
6
Unit gravity sedimentation analysis of basic nuclear protein synthesis during the HeLa cell cycle.海拉细胞周期中碱性核蛋白合成的单位重力沉降分析
In Vitro. 1973 Jan-Feb;8(4):295-300. doi: 10.1007/BF02615909.
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Transcription of simian virus 40. II. Hybridization of RNA extracted from different lines of transformed cells to the separated strands of simian virus 40 DNA.猴病毒40的转录。II. 从不同转化细胞系中提取的RNA与猴病毒40 DNA单链的杂交。
J Virol. 1973 Jul;12(1):90-8. doi: 10.1128/JVI.12.1.90-98.1973.
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Rapid, simultaneous measurement of DNA, protein, and cell volume in single cells from large mammalian cell populations.对来自大型哺乳动物细胞群体的单细胞中的DNA、蛋白质和细胞体积进行快速、同步测量。
J Cell Biol. 1973 Dec;59(3):766-71. doi: 10.1083/jcb.59.3.766.
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The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses.非整倍体中国仓鼠细胞系CHO的染色体:G带、C带及放射自显影分析
Chromosoma. 1973;41(2):129-44. doi: 10.1007/BF00319690.
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Foreign DNA introduced by calcium phosphate is integrated into repetitive DNA elements of the mouse L cell genome.通过磷酸钙导入的外源DNA整合到小鼠L细胞基因组的重复DNA元件中。
Mol Cell Biol. 1986 May;6(5):1787-95. doi: 10.1128/mcb.6.5.1787-1795.1986.