Paciga M, Nurse C A
Department of Biology, McMaster University, 1280 Main Street West Hamilton, Ontario, Canada.
Neuroreport. 2001 Oct 29;12(15):3287-91. doi: 10.1097/00001756-200110290-00028.
Exposure of perinatal rat carotid body (CB) O2-chemoreceptors to basic fibroblast growth factor (bFGF) or hypoxia in vitro increases mitotic activity. Using double-label immunofluorescence, we localized bFGF and its receptor (FGFR) to tyrosine hydroxylase-positive (TH+) chemoreceptors in vitro; bFGF immunoreactivity also localized to chemoreceptors in CB tissue sections. Mitotic activity, measured as percentage TH+ cells that took up bromodeoxyuridine, was relatively constant ( approximately 29%) in normoxic (20% O2) cultures grown with or without bFGF neutralizing antibody (nAb). However, the number of surviving chemoreceptors was significantly reduced in nAb-treated cultures. Under chronic hypoxia (6% O2), the presence of nAb significantly reduced chemoreceptor survival to approximately 70% of control, without affecting mitotic activity. Thus, autocrine/ paracrine actions of endogenous bFGF may help promote CB chemoreceptor survival.
围产期大鼠颈动脉体（CB）的O2化学感受器在体外暴露于碱性成纤维细胞生长因子（bFGF）或缺氧环境会增加有丝分裂活性。我们采用双标记免疫荧光法在体外将bFGF及其受体（FGFR）定位到酪氨酸羟化酶阳性（TH+）化学感受器上；bFGF免疫反应性也定位在CB组织切片中的化学感受器上。以摄取溴脱氧尿苷的TH+细胞百分比来衡量的有丝分裂活性，在添加或不添加bFGF中和抗体（nAb）的常氧（20% O2）培养物中相对恒定（约29%）。然而，在nAb处理的培养物中，存活的化学感受器数量显著减少。在慢性缺氧（6% O2）条件下，nAb的存在显著降低化学感受器存活率至对照的约70%，而不影响有丝分裂活性。因此，内源性bFGF的自分泌/旁分泌作用可能有助于促进CB化学感受器的存活。
