Kageyama M, Shinomiya T, Aihara Y, Kobayashi M
J Virol. 1979 Dec;32(3):951-7. doi: 10.1128/JVI.32.3.951-957.1979.
A bacteriophage with a contractile tail which shows very similar features to R-type pyocins was isolated and characterized. This phage, named PS17,was purified by DEAE-cellulose chromatography and CsCl density gradient centrifugation. It was a DNA-containing phage, and the density of the purified particles in CsCl was found to be 1.468. DNA from this phage had a density of 1.720 in CsCl, indicating its guanine plus cytosine content to be 61.2%. The head was polyhedral, 69 nm in diameter, and the tail was 150 nm in length. This phage was neutralized by antiserum preparations against five R-type pyocins, and the antiserum against this phage was active in neutralizing R-type pyocins. The properties of this phage, PS17, were compared with another similar phage, PS3, which was previously reported.
分离并鉴定了一种具有收缩尾的噬菌体，其表现出与R型绿脓菌素非常相似的特征。这种名为PS17的噬菌体通过DEAE-纤维素色谱法和CsCl密度梯度离心法进行纯化。它是一种含DNA的噬菌体，纯化颗粒在CsCl中的密度为1.468。该噬菌体的DNA在CsCl中的密度为1.720，表明其鸟嘌呤加胞嘧啶含量为61.2%。头部为多面体，直径69nm，尾部长度为150nm。该噬菌体被针对五种R型绿脓菌素的抗血清制剂中和，针对该噬菌体的抗血清在中和R型绿脓菌素方面具有活性。将这种噬菌体PS17的特性与先前报道的另一种相似噬菌体PS3进行了比较。
