Shinomiya T, Shiga S
J Virol. 1979 Dec;32(3):958-67. doi: 10.1128/JVI.32.3.958-967.1979.
The tail of bacteriophage PS17 of Pseudomonas aeruginosa was shown to be bactericidal, and its properties were compared with those of pyocin R1. Temperature-sensitive mutants were isolated from PS17, and the products at nonpermissive temperature were morphologically characterized. Bactericidal substances were found in the lysates of such mutants that were defective in the head formation but not in the tail formation. Phage tails were purified from the lysate of one such mutant, and its chemical and biological properties were studied. Isolated tails killed sensitive cells by a single-hit process and repressed the uptake of leucine in sensitive cells. These results were consistent with the previous findings on the serological and morphological relationship between PS17 and pyocin R1. However, certain differences were also shown between them in shape and protein composition.
铜绿假单胞菌噬菌体PS17的尾部具有杀菌作用，并将其特性与绿脓菌素R1进行了比较。从PS17中分离出温度敏感突变体，并对非允许温度下的产物进行了形态学表征。在头部形成有缺陷但尾部形成无缺陷的此类突变体的裂解物中发现了杀菌物质。从其中一个这样的突变体的裂解物中纯化出噬菌体尾部，并研究了其化学和生物学特性。分离出的尾部通过单次打击过程杀死敏感细胞，并抑制敏感细胞中亮氨酸的摄取。这些结果与之前关于PS17和绿脓菌素R1之间血清学和形态学关系的发现一致。然而，它们在形状和蛋白质组成上也显示出某些差异。
