Buffoni F, Ignesti G
Biochem J. 1975 Feb;145(2):369-72. doi: 10.1042/bj1450369.
Pig plasma benzylamine oxidase is a protein containing cupric copper and pyridoxal phosphate. The pyridoxal phosphate is stably linked to the enzyme. Discrepancies in the numbers of active sites per molecule of enzyme are reported in the literature. This paper shows that the fully active pure enzyme contains 3 mol of pyridoxal phosphate per mol, whereas enzymes with a lower specific activity are shown by titration with phenylhydrazine to have a lower pyrdoxal phosphate content.
猪血浆苄胺氧化酶是一种含有铜离子和磷酸吡哆醛的蛋白质。磷酸吡哆醛与该酶稳定相连。文献报道了每分子酶活性位点数量的差异。本文表明,完全活性的纯酶每摩尔含有3摩尔磷酸吡哆醛,而通过苯肼滴定显示,比活性较低的酶的磷酸吡哆醛含量较低。