用苯肼对猪血浆苄胺氧化酶进行活性-活性滴定。
Active-sitve titration of pig plasma benzylamine oxidase with phenylhydrazine.
作者信息
Buffoni F, Ignesti G
出版信息
Biochem J. 1975 Feb;145(2):369-72. doi: 10.1042/bj1450369.
Abstract
Pig plasma benzylamine oxidase is a protein containing cupric copper and pyridoxal phosphate. The pyridoxal phosphate is stably linked to the enzyme. Discrepancies in the numbers of active sites per molecule of enzyme are reported in the literature. This paper shows that the fully active pure enzyme contains 3 mol of pyridoxal phosphate per mol, whereas enzymes with a lower specific activity are shown by titration with phenylhydrazine to have a lower pyrdoxal phosphate content.
摘要
猪血浆苄胺氧化酶是一种含有铜离子和磷酸吡哆醛的蛋白质。磷酸吡哆醛与该酶稳定相连。文献报道了每分子酶活性位点数量的差异。本文表明，完全活性的纯酶每摩尔含有3摩尔磷酸吡哆醛，而通过苯肼滴定显示，比活性较低的酶的磷酸吡哆醛含量较低。
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本文引用的文献
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PYRIDOXAL PHOSPHATE AS A CONSTITUENT OF THE HISTAMINASE (BENZYLAMINE OXIDASE) OF PIG PLASMA.磷酸吡哆醛作为猪血浆组胺酶（苄胺氧化酶）的一个组成成分
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D-AMINO ACID OXIDASE. I. DISSOCIATION AND RECOMBINATION OF THE HOLOENZYME.D-氨基酸氧化酶。I. 全酶的解离与重组
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MICRODETERMINATION OF MONOAMINE OXIDASE AND 5-HYDROXYTRYPTOPHAN DECARBOXYLASE ACTIVITIES IN NERVOUS TISSUES.神经组织中单胺氧化酶和5-羟色氨酸脱羧酶活性的微量测定
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BENZYLAMINE OXIDASE AND HISTAMINASE: PURIFICATION AND CRYSTALLIZATION OF AN ENZYME FROM PIG PLASMA.苄胺氧化酶和组胺酶：猪血浆中一种酶的纯化与结晶
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MONOAMINE OXIDASE. IV. NATURE OF THE SECOND PROSTHETIC GROUP OF PLASMA MONOAMINE OXIDASE.单胺氧化酶。IV. 血浆单胺氧化酶第二种辅基的性质。
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