Fowke L C, Bech-Hansen C W, Gamborg O L, Constabel F
J Cell Sci. 1975 Aug;18(3):491-507. doi: 10.1242/jcs.18.3.491.
Multinucleate soybean protoplasts produced by spontaneous fusion during enzyme digestion of the cell wall initiated cell division after approximately 40 h in culture. The structure of these protoplasts during mitosis and cytokinesis was studied with both light and electron microscopes. Most nuclei did not fuse but divided synchronously. Interphase nuclei was commonly connected by short narrow nuclear bridges. At prophase and metaphase the nuclei appeared typical of those in most higher plants; technical difficulties prevented an adequate examination of protoplasts at anaphase. Telophase was characterized by cytokinesis involving phragmoplast and cell plate formation; however, complete partitioning of the cytoplasm by cell plants was not observed. Numerous coated vesicles were present near to or continuous with the cell plate and plasmalemma. The presence of a few dividing protoplasts with at least double the normal chromosome number suggests that some nuclear fusion occurred prior to mitosis. Very little cell wall material was detected at the margin of the dividing protoplasts.
在细胞壁酶解过程中通过自发融合产生的多核大豆原生质体，在培养约40小时后开始细胞分裂。利用光学显微镜和电子显微镜研究了这些原生质体在有丝分裂和胞质分裂过程中的结构。大多数细胞核未融合而是同步分裂。间期细胞核通常由短而窄的核桥相连。前期和中期的细胞核表现出大多数高等植物细胞核的典型特征；技术难题阻碍了对后期原生质体进行充分检查。末期的特征是胞质分裂涉及成膜体和细胞板形成；然而，未观察到细胞板对细胞质的完全分隔。许多被膜小泡靠近细胞板或与细胞板及质膜相连。少数分裂原生质体的染色体数目至少是正常染色体数目的两倍，这表明在有丝分裂之前发生了一些核融合。在分裂原生质体的边缘检测到极少的细胞壁物质。
