Department of Biology, University of Saskatchewan, S7N 0W0, Saskatoon, Sask., Canada.
Planta. 1976 Jan;130(1):39-45. doi: 10.1007/BF00390842.
Protoplasts from cultured cells of soybean (Glycine max L.) and from sweet clover (Melilotus officinalis L.) mesophyll cells were fused with polyethylene glycol and subsequently cultured for six days. The resulting fusion products as well as unfused protoplasts of each parental species regenerated cell walls and divided. The fusion products were characterized by the presence of soybean leucoplasts and sweet clover chloroplasts. The chloroplasts appeared to be degenerating but other cytoplasmic organelles were typical of actively growing plant cells. The fate of individual nuclei could not be determined.
用聚乙二醇融合培养的大豆（Glycine max L.）和草木樨（Melilotus officinalis L.）叶肉细胞原生质体，然后在培养 6 天后进行继代培养。所得融合产物以及每一亲本种的未融合原生质体均再生细胞壁并进行分裂。融合产物的特征是存在大豆白色体和草木樨叶绿体。叶绿体似乎在退化，但其他细胞质细胞器是活跃生长的植物细胞的典型特征。无法确定单个核的命运。
