O'Donnell M, Jeruzalmi D, Kuriyan J
The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Curr Biol. 2001 Nov 13;11(22):R935-46. doi: 10.1016/s0960-9822(01)00559-0.
Recent determinations of the crystal structure of the Escherichia coli gamma complex and delta-beta assembly have shed light on the bacterial clamp loading reaction. In this review, we discuss the structures of delta-beta and the gamma(3)deltadelta' complex and its mechanism of action as a clamp loader of the E. coli beta sliding clamp. We also expand upon the implications of the structural findings to the structure and function of the eukaryotic clamp loader, RFC, and the structure of E. coli DNA polymerase III holoenzyme.
最近对大肠杆菌γ复合物和δ-β组装体晶体结构的测定，为细菌钳加载反应提供了线索。在这篇综述中，我们讨论了δ-β和γ(3)δδ'复合物的结构及其作为大肠杆菌β滑动夹钳加载器的作用机制。我们还进一步阐述了这些结构发现对真核钳加载器RFC的结构和功能以及大肠杆菌DNA聚合酶III全酶结构的影响。
