Bernstein E, Denli A M, Hannon G J
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, New York 11724, USA.
RNA. 2001 Nov;7(11):1509-21.
Over the past several years, RNAi and its related phenomena have emerged not only as a powerful experimental tool but also as a new mode of gene regulation. Through a combination of genetic and biochemical approaches we have learned much about the mechanisms underlying dsRNA responses. However, many of the most intriguing aspects of dsRNA-induced gene silencing have yet to be illuminated. What has become abundantly clear is that the complex and highly conserved biology underlying RNA interference is critical both for genome maintenance and for the development of complex organisms. However, it seems probable that we have only begun to reveal the diversity of biological roles played by RNAi-related processes.
在过去几年中,RNA干扰(RNAi)及其相关现象不仅已成为一种强大的实验工具,而且还成为一种新的基因调控模式。通过遗传学和生物化学方法的结合,我们对双链RNA(dsRNA)反应的潜在机制已有了很多了解。然而,dsRNA诱导的基因沉默许多最引人入胜的方面仍有待阐明。已经非常清楚的是,RNA干扰背后复杂且高度保守的生物学对于基因组维持和复杂生物体的发育都至关重要。然而,我们似乎才刚刚开始揭示RNAi相关过程所发挥的生物学作用的多样性。