Gilbert S C
Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Headington, Oxford, OX3 7BN, UK.
Mol Biotechnol. 2001 Oct;19(2):169-77. doi: 10.1385/MB:19:2:169.
Virus-like particles (VLPs) consist of one or more viral coat proteins that assemble into particles. They can be taken up by antigen presenting cells (APC), peptides derived from them are presented on MHC class I molecules at the cell surface, and thereby prime a CD8+ T cell response, either against the particle-forming protein itself (such as Hepatitis B surface antigen) or additional peptide sequences that are produced as fusions with the particle-forming protein. This article describes the preparation of Ty-VLPs in Saccharomyces cerevisiae, a system that can easily be handled in the laboratory or scaled up for manufacture, and is safe in use.
病毒样颗粒（VLPs）由一种或多种组装成颗粒的病毒衣壳蛋白组成。它们可被抗原呈递细胞（APC）摄取，从中衍生的肽呈递在细胞表面的MHC I类分子上，从而引发CD8 + T细胞反应，该反应既可以针对形成颗粒的蛋白质本身（如乙肝表面抗原），也可以针对与形成颗粒的蛋白质融合产生的其他肽序列。本文描述了在酿酒酵母中制备Ty - VLPs的方法，该系统在实验室中易于操作或扩大规模进行生产，并且使用安全。
