吞噬细胞增强因子对正常人多形核白细胞还原亚甲蓝四氮唑的作用。
The effect of Tuftsin on the nitrous blue tetrazolium reduction of normal human polymorphonuclear leukocytes.
作者信息
Spirer Z, Zakuth V, Golander A, Bogair N, Fridkin M
出版信息
J Clin Invest. 1975 Jan;55(1):198-200. doi: 10.1172/JCI107912.
The influence of Tuftsin, the synthetic phagocytosis-stimulating tetrapeptide (L-threonyl-L-lysyl-L-prolyl-L-arginine), on the nitrous blue tetrazolium (NBT) reduction by human polymorphonuclear leukocytes was investigated. It was found that this substance increases the NBT reduction by approximately as much as endotoxin. Other tetrapeptides do not share this property. When Tuftsin analogs are added to the cell suspension and incubated, they prevent the action of both Tuftsin and endotoxin but not of methylene blue. When washed of the analogs, the cells regain the property to be activated by both Tuftsin and endotoxin. It appears that methylene blue on one hand and Tuftsin and endotoxin on the other hand have different sites for their actions. We suggest that whereas methylene blue diffuses into the cell and acts directly upon the hexosemonophosphate shunt activation, Tuftsin and endotoxin appear to act on the cell membrane binding to specific receptors. By treating the cells with Tuftsin analogs, we probably block these receptors.
研究了合成的促吞噬四肽（L-苏氨酰-L-赖氨酰-L-脯氨酰-L-精氨酸）——吞噬细胞增强肽对人多形核白细胞还原亚甲蓝四氮唑（NBT）的影响。发现该物质增加NBT还原的程度与内毒素大致相同。其他四肽则不具备此特性。当将吞噬细胞增强肽类似物加入细胞悬液并孵育时，它们会抑制吞噬细胞增强肽和内毒素的作用，但不会抑制亚甲蓝的作用。当洗去类似物后，细胞又恢复了被吞噬细胞增强肽和内毒素激活的特性。看来，一方面亚甲蓝与另一方面吞噬细胞增强肽和内毒素的作用位点不同。我们认为，亚甲蓝扩散进入细胞并直接作用于磷酸己糖旁路的激活，而吞噬细胞增强肽和内毒素似乎作用于细胞膜并与特定受体结合。通过用吞噬细胞增强肽类似物处理细胞，我们可能阻断了这些受体。
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