Müller J, Botzenhardt U, Lemmel E M
Blut. 1982 Jul;45(1):13-22. doi: 10.1007/BF00320494.
By means of cobra venom factor (CVF) it is demonstrated that the stimulation of hexosemonophosphate shunt (HMPS) of human polymorphonuclear leukocytes (PMN) by zymosan (Z) and dextran sulfate (DS) is caused by at least two models of activation: (a) via activation caused by phagocytosis, (b) via activated alternative pathway of complement activation (APC). Active factors of APC presented with phagocytizable objects strongly enhance activation of PMN. The effect of APC can be observed in serum-containing as well as in serum-free cultures. It can be demonstrated that in serum-free cultures the factors of APC participating in the activation of PMN are supplied by monocytes. By use of synthetic hexapeptide (HP) representing the COOH-terminal sequence of human C3 further evidence is provided that factors of APC are able to activate the PMN.
通过眼镜蛇毒因子（CVF）证明，酵母聚糖（Z）和硫酸葡聚糖（DS）对人多形核白细胞（PMN）的磷酸己糖旁路（HMPS）的刺激是由至少两种激活模型引起的：（a）通过吞噬作用引起的激活，（b）通过补体激活替代途径（APC）的激活。与可吞噬物质一起呈现的APC活性因子强烈增强PMN的激活。在含血清和无血清培养物中均可观察到APC的作用。可以证明，在无血清培养物中，参与PMN激活的APC因子由单核细胞提供。通过使用代表人C3羧基末端序列的合成六肽（HP），进一步证明了APC因子能够激活PMN。
