Russell R R
Microbios. 1978;23(93-94):136-46.
The glycosyltransferases of S. mutans strain Ingbritt have been resolved by SDS-polyacrylamide gel electrophoresis, followed by incubation in the presence of non-ionic detergent to restore enzyme activity. A group of high molecular weight proteins synthesizing glucans has been identified, as well as three distinct fructan-synthesizing activities. The glucan-forming enzymes have been purified by affinity chromatography on insoluble glucan, followed by gel chromatography in SDS, and antiserum to the purified enzymes has shown that they are antigenically identical within serotypes c, e and f, and cross-react strongly with serotype b.
变形链球菌英布里特菌株的糖基转移酶已通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分离，随后在非离子去污剂存在的情况下孵育以恢复酶活性。已鉴定出一组合成葡聚糖的高分子量蛋白质，以及三种不同的合成果聚糖的活性。形成葡聚糖的酶已通过在不溶性葡聚糖上的亲和色谱法进行纯化，随后在十二烷基硫酸钠中进行凝胶色谱法，针对纯化酶的抗血清表明，它们在血清型c、e和f内抗原性相同，并与血清型b发生强烈交叉反应。
