抗变形链球菌6715葡糖基转移酶单克隆抗体的制备
Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715.
作者信息
Furuta T, Nisizawa T, Chiba J, Hamada S
出版信息
Infect Immun. 1983 Aug;41(2):872-5. doi: 10.1128/iai.41.2.872-875.1983.
Abstract
A mouse hybrid cell line secreted monoclonal antibody which reacted specifically with Streptococcus mutans 6715 (serotype g) glucosyltransferase (GTase)-synthesizing water-insoluble glucan and inhibited with enzyme reaction. The antibody was cross-reactive with GTase of serotype d but not with GTase of other serotypes of S. mutans when an enzyme-linked immunosorbent assay was used.
摘要
一种小鼠杂交细胞系分泌的单克隆抗体，可与变形链球菌6715（血清型g）葡糖基转移酶（GTase）特异性反应，该酶可合成水不溶性葡聚糖并抑制酶反应。当使用酶联免疫吸附测定时，该抗体与血清型d的GTase有交叉反应，但与变形链球菌其他血清型的GTase无交叉反应。
相似文献
1
Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715.
Infect Immun. 1983 Aug;41(2):872-5. doi: 10.1128/iai.41.2.872-875.1983.
2
Characterization of monoclonal antibodies against glucosyltransferase synthesizing water-insoluble glucan from Streptococcus sobrinus B13.
J Dent Res. 1990 Feb;69(2):477-82. doi: 10.1177/00220345900690021201.
3
Purification and characterization of cell-associated glucosyltransferase synthesizing water-insoluble glucan from serotype c Streptococcus mutans.
J Gen Microbiol. 1989 Feb;135(Pt 2):335-44. doi: 10.1099/00221287-135-2-335.
4
Serotype-dependent inhibition of glucan synthesis and cell adherence of Streptococcus mutans by antibody against glucosyltransferase of serotype e S. mutans.
Microbiol Immunol. 1979;23(2):61-70. doi: 10.1111/j.1348-0421.1979.tb00442.x.
5
Characterization of an anti-glucosyltransferase serum specific for insoluble glucan synthesis by Streptococcus mutans.
Infect Immun. 1976 Feb;13(2):494-500. doi: 10.1128/iai.13.2.494-500.1976.
6
Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans.
Infect Immun. 1991 Nov;59(11):4161-7. doi: 10.1128/iai.59.11.4161-4167.1991.
7
Monoclonal antibodies specific to Streptococcus mutans GS-5 glucosyltransferase-C inhibit bacterial glucosyltransferase.
Monoclon Antib Immunodiagn Immunother. 2013 Oct;32(5):330-5. doi: 10.1089/mab.2013.0028.
8
Immunological properties of the primer-independent glucosyltransferase of Streptococcus mutans serotypes d and g.
J Gen Microbiol. 1988 May;134(5):1223-7. doi: 10.1099/00221287-134-5-1223.
9
Antibody binding of glucosyltransferase enzyme preparations from homologous and heterologous serotypes of S. mutans.
Adv Exp Med Biol. 1978;107:317-25. doi: 10.1007/978-1-4684-3369-2_36.
10
Evaluation of three individual glucosyltransferases produced by Streptococcus mutans using monoclonal antibodies.
FEMS Microbiol Lett. 1996 Dec 15;145(3):427-32. doi: 10.1111/j.1574-6968.1996.tb08611.x.
引用本文的文献
1
A monoclonal antibody specific to glucosyltransferase B of Streptococcus mutans GS-5 and its glucosyltransferase inhibitory efficiency.
Hybridoma (Larchmt). 2012 Dec;31(6):430-5. doi: 10.1089/hyb.2012.0047.
2
Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12.
Infect Immun. 1985 Aug;49(2):414-6. doi: 10.1128/iai.49.2.414-416.1985.
3
Monoclonal antibodies to the extracellular glucosyltransferases from Streptococcus sobrinus 6715.
Infect Immun. 1987 Aug;55(8):1900-5. doi: 10.1128/iai.55.8.1900-1905.1987.
4
Identification of mutans streptococci with monoclonal antibodies.
Antonie Van Leeuwenhoek. 1990 Nov;58(4):219-25. doi: 10.1007/BF00399332.
本文引用的文献
1
Biology, immunology, and cariogenicity of Streptococcus mutans.
Microbiol Rev. 1980 Jun;44(2):331-84. doi: 10.1128/mr.44.2.331-384.1980.
2
Immunochemical properties of glucosyltransferases from Streptococcus mutans.
Infect Immun. 1983 Feb;39(2):762-6. doi: 10.1128/iai.39.2.762-766.1983.
3
A high resolution PAS stain for polyacrylamide gel electrophoresis.
Anal Biochem. 1973 Dec;56(2):361-9. doi: 10.1016/0003-2697(73)90202-9.
4
5
Production of large amounts of antibodies in individual mice.
J Immunol. 1976 Mar;116(3):676-81.
6
Continuous cultures of fused cells secreting antibody of predefined specificity.
Nature. 1975 Aug 7;256(5517):495-7. doi: 10.1038/256495a0.
7
Immunological relationships between glucosyltransferases from Streptococcus mutans serotypes.
Infect Immun. 1976 Sep;14(3):636-44. doi: 10.1128/iai.14.3.636-644.1976.
8
Serum glucosyltransferase-inhibiting antibodies and dental caries in rhesus monkeys immunized against Streptococcus mutans.
Immunology. 1976 May;30(5):619-27.
9
Preparation of glucosyltransferase from Streptococcus mutans by elution from water-insoluble polysaccharide with a dissociating solvent.
Infect Immun. 1979 Feb;23(2):446-52. doi: 10.1128/iai.23.2.446-452.1979.
10
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4. doi: 10.1073/pnas.76.9.4350.
Zotero 插件