Das S, Ramm M, Kochanowski H, Basu S
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta.
J Bacteriol. 1994 Nov;176(21):6550-7. doi: 10.1128/jb.176.21.6550-6557.1994.
The lipopolysaccharide (LPS) was isolated from Pseudomonas syringae pv. coriandricola W-43 by hot phenol-water extraction. Rhamnose and 3-N-acetyl-3-deoxyfucose were found to be the major sugar constituents of the LPS together with N-acetylglucosamine, N-acetylgalactosamine, heptose, and 3-deoxy-D-manno-octulosonic acid (Kdo). The main fatty acids of lipid A of the LPS were 3-OH-C:10, C12:0, 2-OH-C12:0, and 3-OH-C12:0. The O-specific polysaccharide liberated from the LPS by mild-acid hydrolysis was purified by gel permeation chromatography. The compositional analysis of the O-specific polysaccharide revealed the presence of L-rhamnose and 3-N-acetyl-3-deoxy-D-fucose in a molar ratio of 4:1. The primary structure of the O-specific polysaccharide was established by methylation analysis together with 1H and 13C nuclear magnetic resonance spectroscopy, including two-dimensional shift-correlated and one-dimensional nuclear Overhauser effect spectroscopy. The polysaccharide moiety was found to consist of a tetrasaccharide rhamnan backbone, and 3-N-acetyl-3-deoxy-D-fucose constitutes the side chain of the branched pentasaccharide repeating unit of the polysaccharide.
通过热酚水萃取法从丁香假单胞菌香菜致病变种W-43中分离出脂多糖（LPS）。发现鼠李糖和3-N-乙酰基-3-脱氧岩藻糖是LPS的主要糖类成分，此外还有N-乙酰葡糖胺、N-乙酰半乳糖胺、庚糖和3-脱氧-D-甘露辛酮糖酸（Kdo）。LPS的类脂A的主要脂肪酸为3-羟基-C:10、C12:0、2-羟基-C12:0和3-羟基-C12:0。通过温和酸水解从LPS释放出的O-特异性多糖经凝胶渗透色谱法纯化。O-特异性多糖的组成分析显示L-鼠李糖和3-N-乙酰基-3-脱氧-D-岩藻糖的摩尔比为4:1。通过甲基化分析以及1H和13C核磁共振光谱法（包括二维位移相关光谱法和一维核Overhauser效应光谱法）确定了O-特异性多糖的一级结构。发现多糖部分由一个四糖鼠李聚糖主链组成，3-N-乙酰基-3-脱氧-D-岩藻糖构成多糖支链五糖重复单元的侧链。
