Plaggenborg R, Steinbüchel A, Priefert H
Institut für Mikrobiologie der Westfälischen Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Münster, Germany.
FEMS Microbiol Lett. 2001 Nov 27;205(1):9-16. doi: 10.1111/j.1574-6968.2001.tb10918.x.
The gene loci fcs and ech, encoding feruloyl-CoA synthetase and enoyl-CoA hydratase/aldolase, respectively, are involved in the ferulic acid catabolism in Delftia acidovorans. The amino acid sequence deduced from ech exhibited 51% identity to the enoyl-CoA hydratase/aldolase from Pseudomonas sp. strain HR199, indicating that the enzyme from D. acidovorans represents a new lineage of this protein. The genes fcs and ech were expressed in Escherichia coli enabling the recombinant strain to transform ferulic acid to vanillin as revealed by photometric and HPLC analysis. An fcs deficient mutant of D. acidovorans was unable to grow on ferulic acid. The obtained data suggest that in contrast to a previous publication the biotechnologically interesting direct non-oxidative deacetylation mechanism of ferulic acid cleavage is not realized in D. acidovorans. Instead, ferulic acid degradation in D. acidovorans proceeds via a coenzyme A-dependent non-beta-oxidative pathway.
分别编码阿魏酰辅酶A合成酶和烯酰辅酶A水合酶/醛缩酶的基因位点fcs和ech，参与了食酸代尔夫特菌中阿魏酸的分解代谢。从ech推导的氨基酸序列与假单胞菌属菌株HR199的烯酰辅酶A水合酶/醛缩酶有51%的同一性，这表明食酸代尔夫特菌的该酶代表了这种蛋白质的一个新谱系。基因fcs和ech在大肠杆菌中表达，通过光度分析和高效液相色谱分析表明，重组菌株能够将阿魏酸转化为香草醛。食酸代尔夫特菌的fcs缺陷型突变体无法在阿魏酸上生长。获得的数据表明，与之前的一篇出版物相反，食酸代尔夫特菌中未实现具有生物技术意义的阿魏酸裂解直接非氧化脱乙酰化机制。相反，食酸代尔夫特菌中的阿魏酸降解是通过辅酶A依赖性非β氧化途径进行的。
