Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands.
Appl Environ Microbiol. 2011 Jan;77(2):572-9. doi: 10.1128/AEM.00393-10. Epub 2010 Nov 19.
A consortium of the newly isolated bacterial strains Arthrobacter sp. strain G1 and Ralstonia sp. strain H1 utilized 4-fluorocinnamic acid for growth under aerobic conditions. Strain G1 converted 4-fluorocinnamic acid into 4-fluorobenzoic acid and used the two-carbon side chain for growth, with some formation of 4-fluoroacetophenone as a dead-end side product. In the presence of strain H1, complete mineralization of 4-fluorocinnamic acid and release of fluoride were obtained. Degradation of 4-fluorocinnamic acid by strain G1 occurred through a β-oxidation mechanism and started with the formation of 4-fluorocinnamoyl-coenzyme A (CoA), as indicated by the presence of 4-fluorocinnamoyl-CoA ligase. Enzymes for further transformation were detected in cell extract, i.e., 4-fluorocinnamoyl-CoA hydratase, 4-fluorophenyl-β-hydroxy propionyl-CoA dehydrogenase, and 4-fluorophenyl-β-keto propionyl-CoA thiolase. Degradation of 4-fluorobenzoic acid by strain H1 proceeded via 4-fluorocatechol, which was converted by an ortho-cleavage pathway.
由新分离的细菌菌株节杆菌 G1 菌株和罗尔斯顿氏菌 H1 菌株组成的联合体在需氧条件下利用 4-氟肉桂酸进行生长。菌株 G1 将 4-氟肉桂酸转化为 4-氟苯甲酸，并利用双碳侧链进行生长，同时形成一些 4-氟苯乙酮作为末端副产物。在菌株 H1 的存在下，可实现 4-氟肉桂酸的完全矿化和氟的释放。菌株 G1 通过β-氧化机制降解 4-氟肉桂酸，首先形成 4-氟肉桂酰辅酶 A（CoA），这表明存在 4-氟肉桂酰辅酶 A 连接酶。在细胞提取物中检测到进一步转化的酶，即 4-氟肉桂酰辅酶 A 水合酶、4-氟苯基-β-羟基丙酰辅酶 A 脱氢酶和 4-氟苯基-β-酮丙酰辅酶 A 硫解酶。菌株 H1 通过 4-氟邻苯二酚降解 4-氟苯甲酸，该过程通过邻位裂解途径进行。
