Koebnik R
Martin-Luther-Universität, Institut für Genetik, Weinbergweg 10, D-06120, Halle (Saale), Germany.
Trends Microbiol. 2001 Dec;9(12):586-90. doi: 10.1016/s0966-842x(01)02255-7.
Gram-negative bacteria have surface appendages that assemble via different secretion machineries. Recently, new experimental approaches have contributed to a better understanding of the molecular mechanisms of flagellar and pilus assembly, and protein secretion. These findings can be applied to plant pathogenic bacteria, which probably transfer effector proteins directly into their eukaryotic host cells. Here, it is suggested that assembly of Hrp pili occurs in the periplasm and that unfolded effector proteins attach to pilins within the pili, thus effecting protein translocation. A two-domain structure for the HrpA pilin from Pseudomonas syringae is also predicted.
革兰氏阴性菌具有通过不同分泌机制组装的表面附属物。最近，新的实验方法有助于更好地理解鞭毛和菌毛组装以及蛋白质分泌的分子机制。这些发现可应用于植物致病细菌，它们可能将效应蛋白直接转移到其真核宿主细胞中。在此，有人提出Hrp菌毛的组装发生在周质中，未折叠的效应蛋白附着在菌毛内的菌毛蛋白上，从而实现蛋白质转运。还预测了丁香假单胞菌HrpA菌毛蛋白的双结构域结构。
