Arakawa T, Wen J
Alliance Protein Laboratories, 3957 Corte Cancion, Thousand Oaks, California 91360, USA.
Anal Biochem. 2001 Dec 15;299(2):158-61. doi: 10.1006/abio.2001.5432.
In the light scattering technique, glycosylation gives rise to excess light scattering for glycoproteins. Assuming additivity of refractive index and using an appropriate refractive index increment for carbohydrate, one can determine the degree of glycosylation from the excess light scattering. Here we have used size-exclusion chromatography in combination with online light scattering, UV absorbance, and refractive index. The results show that the technique accurately determines the carbohydrate content of recombinant stem cell factor.
在光散射技术中，糖基化会导致糖蛋白产生过量的光散射。假设折射率具有加和性，并使用合适的碳水化合物折射率增量，就可以从过量的光散射中确定糖基化程度。在这里，我们将尺寸排阻色谱与在线光散射、紫外吸光度和折射率相结合。结果表明，该技术能够准确测定重组干细胞因子的碳水化合物含量。
