Verjans H L, van der Molen H J, Eik-Nes K B
Acta Endocrinol (Copenh). 1975 Jun;79(2):380-6. doi: 10.1530/acta.0.0790380.
Serum levels of LH, FSH and testosterone were measured by radioimmunoassay in intact and castrated, adult, male rats after testosterone was administered subcutaneously for seven days in doses ranging from 25 to 200 mug per 100 g body weight per day. Such treatment increased circulating testosterone both in intact and castrated rats, but its effects on serum gonadotrophins were different in these animal groups. All doses of testosterone suppressed serum LH and FSH in the normal rat. In the castrates, treatment with the lowest dose of testosterone resulted in serum LH levels significantly above the high castrate levels, while serum FSH tended to drop. Administration of the highest doses of testosterone did not depress serum LH and FSH in the castrates to those of intact, normal animals, though serum testosterone in these castrates was much higher than in normal, male rats. It is concluded, that the sensitivity of the hypothalamic-pituitary system for daily, subcutaneous testosterone administration during seven days is not the same in the intact and castrated, adult, male rat and that testicular factors different from testosterone may play a role in regulating production and/or secretion of gonadotrophins by the hypophysis in male animals.
通过放射免疫分析法，测定了成年雄性大鼠完整组和去势组在每天按每100克体重25至200微克的剂量皮下注射睾酮7天后血清促黄体生成素（LH）、促卵泡生成素（FSH）和睾酮的水平。这种处理使完整大鼠和去势大鼠的循环睾酮水平均升高，但对这些动物组血清促性腺激素的影响不同。所有剂量的睾酮均抑制正常大鼠的血清LH和FSH。在去势大鼠中，用最低剂量的睾酮处理导致血清LH水平显著高于高去势水平，而血清FSH则趋于下降。给予最高剂量的睾酮并没有使去势大鼠的血清LH和FSH降至完整正常动物的水平，尽管这些去势大鼠的血清睾酮水平远高于正常雄性大鼠。得出的结论是，成年雄性大鼠完整组和去势组在连续7天每天皮下注射睾酮时，下丘脑 - 垂体系统的敏感性不同，并且不同于睾酮的睾丸因素可能在调节雄性动物垂体促性腺激素的产生和/或分泌中起作用。
