Petersen E, Boman J, Wågberg F, Bergström S, Angquist K A
Department of Surgery, University Hospital, S-90185 Umeå, Sweden.
Eur J Vasc Endovasc Surg. 2001 Nov;22(5):443-7. doi: 10.1053/ejvs.2001.1489.
to investigate whether Chlamydia pneumoniae (C. pneumoniae) may increase elastin degradation in the aortic wall.
eighteen full thickness aortic wall samples from non-aneurysmal infrarenal abdominal aortas were collected from autopsies. Two adjacent and equally large pieces were cut out of each aortic sample. From each sample, one piece was incubated in a HEp-2 cell culture infected with C. pneumoniae and the other piece was incubated in an uninfected HEp-2 cell culture. The incubation time was one week at 35 degrees C. The concentration of elastin-derived peptides (EDP) (ng/ml) in the medium of each cell culture was measured in duplicate. For each paired sample, delta-EDP (EDP in HEp-2 cell culture infected with C. pneumoniae- EDP in uninfected HEp-2 cell culture) was calculated.
there was a significantly increased degradation of aortic elastin, estimated by EDP concentrations in cell culture conditioned medium, when aortic wall samples were incubated in C. pneumoniae cultures compared with uninfected cultures (p=0.025, Wilcoxon signed ranks test).
these results indicate that there is a relationship between the presence of C. pneumoniae and increased elastin degradation in the aortic wall in vitro. This suggests C. pneumoniae in the aortic wall directly or indirectly leads to the degradation of aortic elastin.
研究肺炎衣原体（C. pneumoniae）是否会增加主动脉壁中弹性蛋白的降解。
从尸检中收集18个来自非动脉瘤性肾下腹主动脉的全层主动脉壁样本。从每个主动脉样本中切出两块相邻且大小相同的组织块。从每个样本中，一块在感染肺炎衣原体的HEp-2细胞培养物中孵育，另一块在未感染的HEp-2细胞培养物中孵育。孵育时间为在35摄氏度下一周。对每个细胞培养物培养基中弹性蛋白衍生肽（EDP）（ng/ml）的浓度进行重复测量。对于每对样本，计算Δ-EDP（感染肺炎衣原体的HEp-2细胞培养物中的EDP - 未感染的HEp-2细胞培养物中的EDP）。
与未感染的培养物相比，当主动脉壁样本在肺炎衣原体培养物中孵育时，通过细胞培养条件培养基中的EDP浓度估计，主动脉弹性蛋白的降解显著增加（p = 0.025，Wilcoxon符号秩检验）。
这些结果表明，肺炎衣原体的存在与体外主动脉壁中弹性蛋白降解增加之间存在关联。这表明主动脉壁中的肺炎衣原体直接或间接导致主动脉弹性蛋白的降解。
