Department of Medical Sciences, Infectious Diseases, Uppsala University Hospital, Uppsala University, Uppsala, Sweden.
Ups J Med Sci. 2010 Aug;115(3):181-6. doi: 10.3109/03009731003778719.
Chlamydophila pneumoniae (C. pneumoniae) has been associated with atherosclerosis and abdominal aortic aneurysm and is probably disseminated by peripheral blood mononuclear cells (PBMC). Viable and metabolically active bacteria can be demonstrated by the presence of bacterial mRNA and on-going dissemination by the presence of bacteria in PBMC. The aim of this study was to determine the prevalence of C. pneumoniae DNA and mRNA in aortic biopsies and C. pneumoniae DNA in PBMC in thoracic aortic aneurysm and aortic dissection patients.
Real-time PCR was used to detect C. pneumoniae DNA and mRNA in biopsies and C. pneumoniae DNA in PBMC.
C. pneumoniae DNA was found in biopsies in 26% (6/23) of aneurysm patients and 11% (2/18) of dissection patients but in none of the forensic autopsy controls. C. pneumoniae mRNA was not found in any biopsy, and all PBMC were C. pneumoniae-negative.
Presence of C. pneumoniae DNA but not mRNA in aortic biopsies and no evidence of C. pneumoniae in PBMC suggest that the infection in the aorta has passed into a state of persistence.
肺炎衣原体（C. pneumoniae）与动脉粥样硬化和腹主动脉瘤有关，可能通过外周血单核细胞（PBMC）传播。通过存在细菌 mRNA 可以证明活菌和代谢活跃的细菌，并且通过 PBMC 中存在细菌可以证明正在进行传播。本研究旨在确定胸主动脉瘤和主动脉夹层患者的主动脉活检中肺炎衣原体 DNA 和 mRNA 以及 PBMC 中肺炎衣原体 DNA 的患病率。
实时 PCR 用于检测活检中的肺炎衣原体 DNA 和 mRNA 以及 PBMC 中的肺炎衣原体 DNA。
在 23 例动脉瘤患者中有 26%（6/23）和 18 例夹层患者中有 11%（2/18）发现肺炎衣原体 DNA，但在法医尸检对照中均未发现。在任何活检中均未发现肺炎衣原体 mRNA，并且所有 PBMC 均为肺炎衣原体阴性。
主动脉活检中存在肺炎衣原体 DNA 但不存在 mRNA，并且 PBMC 中没有肺炎衣原体的证据表明主动脉中的感染已进入持续状态。
