Legrand E, Kressmann M C, Duplan J F
Int J Cancer. 1975 Apr 15;15(4):572-87. doi: 10.1002/ijc.2910150407.
Luekaemogenesis and repopulation of the lymphoid system have been studied in sub-lethally irradiated (c3h x akr/t1ald) f1 hybrids which have been restored with parental Bone Marrow (BM) cells with or without thymic cells. The metacentric marker of the T1ALD sub-strain made it possible to identify the host or donor orgin of leukaemias. Leukaemias occur either in the thymuc (lymphosarcomas) or in the other lymphoid tissues (extra-thymic leukaemias). After irradiation, the precentage of lymphosarcomas increases from 5 to 95%. The rate of leukaemias in hybrids which have been restored only with BM cells is 55, 56 and 100% respectively depending on the origin of BM: syngenic, C3H and AKR. In this last group 75% of the lymphosarcomas originate from donor cells. The inhibitory efficiency of the three kinds of BM on luekaemogenesis seems to be related to their respective abilities to spontaneous malignant transformation. When AKR or C3H thymic cells are injected together with BM cells, leukaemogenesis is altered. The effect is indirect as these cells are not actually concerned by the malignant transformation. The percentage of LS is significantly reduced and the mean survival improved in hybrids restored with C3H thymic, mixed AKR or C3H BM cells. AKR thymic cells are less efficient. In both cases, the percentage of extra thymic leukaemias increases at the expense of lymphosarcomas. Thymic cells do not change the kinetics of repopulation in thymus and lymph-node by the BM cells, except when C3H thymic cells are mixed with T1ALD BM cells; in this case, the lymph-node repopulation is temporarily enhanced. Different hypotheses might explain the effect of thymic cells on leukaemogenesis: enhanced recovery of the postirradiation immunological deficiency, transfer of virus by the AKR thymic cells, and more probably influence of the thymic cells on the maturation or/and differentiation of the lymphoid cells.
在接受亚致死剂量照射的（C3H×AKR/T1ALD）F1杂交种中研究了淋巴系统的白血病发生和再填充情况，这些杂交种已用亲代骨髓（BM）细胞进行了恢复，有的还添加了胸腺细胞。T1ALD亚系的中着丝粒标记使得能够识别白血病的宿主或供体来源。白血病发生在胸腺（淋巴肉瘤）或其他淋巴组织（胸腺外白血病）中。照射后，淋巴肉瘤的百分比从5%增加到95%。仅用BM细胞恢复的杂交种中白血病的发生率分别为55%、56%和100%，这取决于BM的来源：同基因、C3H和AKR。在最后一组中，75%的淋巴肉瘤起源于供体细胞。三种BM对白血病发生的抑制效率似乎与其自发恶性转化的各自能力有关。当AKR或C3H胸腺细胞与BM细胞一起注射时，白血病发生会改变。这种作用是间接的，因为这些细胞实际上与恶性转化无关。用C3H胸腺细胞、混合的AKR或C3H BM细胞恢复的杂交种中，LS的百分比显著降低，平均生存期延长。AKR胸腺细胞的效果较差。在这两种情况下，胸腺外白血病的百分比增加，以牺牲淋巴肉瘤为代价。胸腺细胞不会改变BM细胞在胸腺和淋巴结中的再填充动力学，除非C3H胸腺细胞与T1ALD BM细胞混合；在这种情况下，淋巴结的再填充会暂时增强。不同的假说来解释胸腺细胞对白血病发生的作用：照射后免疫缺陷的恢复增强、AKR胸腺细胞转移病毒，更可能的是胸腺细胞对淋巴细胞成熟或/和分化的影响。
