精浆α-葡萄糖苷酶酶指标对精子活力和受精能力的诊断价值。

The diagnostic value of seminal a-glucosidase enzyme index for sperm motility and fertilizing capacity.

作者信息

Yassa D A, Idriss W K, Atassi M E, Rao S K

机构信息

Arab Fertility Center, Khalid Idriss Hospital, Jeddah 21411, Kingdom of Saudi Arabia.

出版信息

Saudi Med J. 2001 Nov;22(11):987-91.

PMID:11744971

Abstract

OBJECTIVE

To find out the diagnostic value of seminal a-glucosidase enzyme index in the ejaculated semen samples of proven-fertility and primary infertility patients, for the sperm motility and fertilizing capacity.

METHODS

The study which lasted approximately 2 years was carried out in the Andrology Department of the Arab Fertility Center, Jeddah, Kingdom of Saudi Arabia. Eighty-five healthy male patients (age 25-38 years) were admitted into the study. They were divided into 2 main groups as proven-fertility group and primary infertility group. Each group was again subdivided into 4 classes based on the sperm concentrations. The sperm motility assessment was carried out quantitatively by Semi automated Computer assisted Semen analysis using Autosperm analyzer. Simultaneously, a-glucosidase enzyme index was determined in all the semen samples.

RESULTS

The results were tabulated as per the standard sperm motility grades of the World Health Organisation, under the classified sperm concentrations of the study. The progressive motility grades were compared with the enzyme index of a-glucosidase in both the groups. The total progressive motility was found higher in the high-density (classes I and II) than the low-density (classes III and IV) semen samples. The enzyme index readings of the various classes of sperm concentrations were significant (p<0.05) and showed a stepwise fall from 89% +/-9% to 52%+/-3% in the proven fertility group. Although, a similar trend has been observed (48%+/-5% to 20%+/-2%) in the primary infertility group, the enzyme index class II sperm concentration (44%+/-6%) was not found significant with class 1 (48%+/-5%) sperm concentration, but the significance was achieved with the other 2 classes. The readings of enzyme index between the groups were found highly significant (p, 0.001).

CONCLUSION

The enzyme index of a-glucosidase estimations in semen samples may indicate a positive correlation with progressive motility and fertilizing capacity of spermatozoa and may serve as diagnostic value in individuals showing normal levels of sperm concentration and serum androgen.

摘要

目的

探讨精浆α-葡萄糖苷酶指标对已证实有生育能力和原发性不育患者精液样本中精子活力及受精能力的诊断价值。

方法

该研究在沙特阿拉伯王国吉达市阿拉伯生育中心男科进行，为期约2年。85名健康男性患者（年龄25 - 38岁）纳入研究。他们被分为两个主要组，即已证实有生育能力组和原发性不育组。每组又根据精子浓度细分为4类。使用Autosperm分析仪通过半自动计算机辅助精液分析对精子活力进行定量评估。同时，测定所有精液样本中的α-葡萄糖苷酶指标。

结果

根据世界卫生组织的标准精子活力等级，在研究分类的精子浓度下将结果制成表格。比较两组中渐进性活力等级与α-葡萄糖苷酶的指标。发现高密度（I类和II类）精液样本中的总渐进性活力高于低密度（III类和IV类）精液样本。在已证实有生育能力组中，各类精子浓度的酶指标读数具有显著性（p<0.05），并显示从89%±9%逐步下降至52%±3%。虽然在原发性不育组中观察到类似趋势（48%±5%至20%±2%），但酶指标II类精子浓度（44%±6%）与I类（48%±5%）精子浓度相比无显著性差异，但与其他两类有显著性差异。两组间酶指标读数差异极显著（p<0.001）。