Herzig R H, Herzig G P, Bull M I, Decter J A, Lohrmann H P, Stout F G, Yankee R A, Graw R G
Blood. 1975 Nov;46(5):743-50.
Matching donor-recipient pairs for HL-A antigens provides a logical starting point for selecting donors for recipients with extensive prior transfusion histories. However, during the course of continued exposure to even HL-A-matched platelet concentrates, further sensitization occurs, as indicated by progressively poorer post-transfusion increments and transfusion reactions. There is evidence that such sensitization may be due to non-HL-A antigens. Finally, it is postulated that the poor post-transfusion platelet increments obtained when standard platelet concentrates are used result from the leukoagglutinin antigen-antibody reaction involving the platelet as an "innocent bystander." The standard platelet concentrate can be purified by a simple method of centrifugation (178 g times 3 min), removing about 96% of the contaminating white blood cells with concomitant loss of about 21% of the platelets. The use of these leukocyte-poor platelet concentrates can restore compatible transfusion increments in highly alloimmunized thrombocytopenic recipients. The luekocyte-poor concentrates can diminish undesirable transfusion reactions following imcompatible platelet transfusions.
为具有广泛既往输血史的受者选择供者时，匹配供受者的人类白细胞抗原（HL-A）可作为一个合理的起点。然而，即使在持续输注HL-A匹配的血小板浓缩物过程中，仍会发生进一步的致敏，表现为输血后血小板增加逐渐减少以及出现输血反应。有证据表明，这种致敏可能归因于非HL-A抗原。最后，有人推测，使用标准血小板浓缩物时输血后血小板增加不佳是由于涉及血小板作为“无辜旁观者”的白细胞凝集素抗原-抗体反应所致。标准血小板浓缩物可通过简单的离心方法（178g×3分钟）进行纯化，可去除约96%的污染白细胞，同时约21%的血小板会随之损失。使用这些少白细胞血小板浓缩物可使高度同种免疫的血小板减少症受者恢复相容性输血后的血小板增加。少白细胞浓缩物可减少不相容血小板输血后的不良输血反应。
