Li Y F, Yuan L, Xu Y K, Yang M, Zhao Y M, Luo Z P
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
Acta Pharmacol Sin. 2001 Dec;22(12):1084-8.
To explore the antistress effect of Morinda officinalis (Chinese medicine "Bajitian") oligosaccharides (MW-97) in mice.
Male mice and rats were subjected to a variety of unpredictable stressors on a daily basis over 15 d period and then the Vidiomex-V image pattern analytic system was used to observe the spontaneous motor activity. Meanwhile, regular method was used to prepare pathologic section of adrenal gland and blood cells analytic system was used to detect white blood cell (WBC) count (including relative WBC percentages) in peripheral blood. On the end, the serum level of stress hormone was detected using radioimmunoassay.
Chronic stress resulted in diffuse hyperplasia of the adrenal cortex and atrophy of the adrenal medulla in mice, which suggested that stress-adaption failure of the adrenal gland occurred, while adrenal gland of the mice pretreated with MW-97 (100 mg/kg, ip) prior to each stressor for 15 d did not occur any pathologic changes. In addition, chronic stress also significantly reduced the WBC count and relative WBC percentages in the peripheral blood, including the percentage of lymphocytes, monocytes, neutrophils, however, MW-97 (25 and 100 mg/kg) reversed these changes and raised WBC count, along with relative WBC percentages significantly. Furthermore, the serum concentration of testosterone was decreased and corticosterone was increased significantly in chronically stressed animals. MW-97 also declined the serum level of corticosterone and raised level of testosterone. MW-97 had no effects on the spontaneous motor activity in the stressed mice.
MW-97 had antistress effect against chronic stress, moreover, MW-97 had no excitatory or inhibitory effects on the CNS, which suggested that MW-97 might become a new kind of antistress agent.
探讨巴戟天寡糖（MW - 97）对小鼠的抗应激作用。
雄性小鼠和大鼠在15天内每日接受多种不可预测的应激源刺激，然后使用Vidiomex - V图像模式分析系统观察自发运动活动。同时，采用常规方法制备肾上腺病理切片，并用血细胞分析系统检测外周血白细胞（WBC）计数（包括相对WBC百分比）。最后，采用放射免疫分析法检测应激激素的血清水平。
慢性应激导致小鼠肾上腺皮质弥漫性增生和肾上腺髓质萎缩，提示肾上腺出现应激适应失败，而在每次应激源刺激前15天用MW - 97（100 mg/kg，腹腔注射）预处理的小鼠肾上腺未出现任何病理变化。此外，慢性应激还显著降低外周血白细胞计数和相对WBC百分比，包括淋巴细胞、单核细胞、中性粒细胞的百分比，然而，MW - 97（25和100 mg/kg）可逆转这些变化并显著提高白细胞计数和相对WBC百分比。此外，慢性应激动物血清睾酮浓度降低，皮质酮显著升高。MW - 97也降低了皮质酮的血清水平并提高了睾酮水平。MW - 97对应激小鼠的自发运动活动无影响。
MW - 97对慢性应激具有抗应激作用，而且MW - 97对中枢神经系统无兴奋或抑制作用，这表明MW - 97可能成为一种新型抗应激剂。
