Rijneveld Anita W, Lauw Fanny N, Schultz Marc J, Florquin Sandrine, Te Velde Anje A, Speelman Peter, Van Deventer Sander J H, Van Der Poll Tom
Department of Experimental Internal Medicine, Tropical Medicine, and AIDS, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
J Infect Dis. 2002 Jan 1;185(1):91-7. doi: 10.1086/338122. Epub 2001 Dec 14.
To determine the role of interferon (IFN)-gamma in pneumonia, IFN-gamma receptor-deficient (IFN-gamma R(-/-)) and 129/Sv (wild-type [wt]) mice were inoculated intranasally with Streptococcus pneumoniae. Although mortality did not differ between the groups 48 h after inoculation, IFN-gamma R(-/-) mice had significantly fewer pneumococci in their lungs than the wt mice. Similarly, IFN-gamma(-/-) mice had fewer colony-forming units in lungs than wt mice. The relatively increased resistance of IFN-gamma R(-/-) mice was not related to favorable effects on defense mechanisms known to contribute to antibacterial immunity-that is, the neutrophilic influx was reduced and the cytokine and nitric oxide levels were similar or lower in IFN-gamma R(-/-) mice. In contrast, mice treated with anti-IFN-gamma did not demonstrate a consistently altered bacterial outgrowth, compared with mice treated with a control antibody. These data suggest that endogenous IFN-gamma, despite its protective role in defense against intracellular pathogens, does not serve a protective role during pneumococcal pneumonia.
为了确定干扰素（IFN）-γ在肺炎中的作用，将缺乏IFN-γ受体（IFN-γR(-/-)）的小鼠和129/Sv（野生型[wt]）小鼠经鼻接种肺炎链球菌。尽管接种后48小时两组之间的死亡率没有差异，但IFN-γR(-/-)小鼠肺中的肺炎球菌明显少于野生型小鼠。同样，IFN-γ(-/-)小鼠肺中的菌落形成单位也比野生型小鼠少。IFN-γR(-/-)小鼠相对增强的抵抗力与对已知有助于抗菌免疫的防御机制的有利影响无关，也就是说，IFN-γR(-/-)小鼠的中性粒细胞流入减少，细胞因子和一氧化氮水平相似或更低。相比之下，与用对照抗体处理的小鼠相比，用抗IFN-γ处理的小鼠并未表现出细菌生长的持续改变。这些数据表明，内源性IFN-γ尽管在抵御细胞内病原体方面具有保护作用，但在肺炎球菌肺炎期间并不起保护作用。
