无证据表明麻风菌素反应与NRAMP1基因座之间存在连锁关系。

No evidence of linkage between Mitsuda reaction and the NRAMP1 locus.

作者信息

Hatagima A, Opromolla D V, Ura S, Feitosa M F, Beiguelman B, Krieger H

机构信息

Departmento de Genetica, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.

出版信息

Int J Lepr Other Mycobact Dis. 2001 Jun;69(2):99-103.

PMID:11757172

Abstract

Thirty sib-pairs were ascertained through unrelated lepromatous probands. They consisted of 22 healthy individuals and 8 leprosy patients. The Mitsuda reactions of all sibs were evaluated both macroscopically and histologically, and high molecular weight genomic DNA was extracted from the white blood cells of all sib-pairs. Three DNA polymorphisms identified by polymerase chain reaction (274C/T, D543N, 1729 + 55del4) were used as chromosome markers at the NRAMP1 locus. Sib-pair comparisons did not disclose any sign of close linkage between the Mitsuda reaction and the genetic markers.

摘要

通过无亲缘关系的瘤型麻风先证者确定了30对同胞对。他们包括22名健康个体和8名麻风病患者。对所有同胞的麻风菌素反应进行了宏观和组织学评估，并从所有同胞对的白细胞中提取了高分子量基因组DNA。通过聚合酶链反应鉴定的三个DNA多态性（274C/T、D543N、1729 + 55del4）被用作NRAMP1基因座的染色体标记。同胞对比较未发现麻风菌素反应与遗传标记之间存在紧密连锁的任何迹象。