Petri V, Mendes E V, Beiguelman B
Int J Lepr Other Mycobact Dis. 1985 Dec;53(4):540-5.
Mitsuda tests were performed on 100 healthy adult Brazilian males unrelated consanguineously to leprosy patients and with no known contacts with this disease. Three lepromatous patients served as negative Mitsuda controls. The lepromin employed was from Mycobacterium leprae-infected armadillo tissue prepared according to WHO standards. Clinical response to the lepromin inoculation was graded according to the recommendations of the VI International Leprosy Congress held in Madrid (1953). The histological reaction was graded according to the authors' criteria based on the intensity of the granulomatous response. The clinically positive (77), the doubtful (19), and 1 out of 4 clinically negative Mitsuda reactors disclosed histologically positive reactions of different degrees of intensity. A significant association between the clinical and histological readings of the Mitsuda reaction was demonstrated when the results of both readings were grouped in two classes: negative and non-negative. However, the different degrees of the histologically positive lepromin reactions were distributed at random among the clinically positive Mitsuda reactors. The frequency of histologically negative Mitsuda reactors observed among the healthy subjects who had no known contacts with leprosy patients (3%) was significantly low when compared to the frequencies reported in the pertinent literature concerning healthy contacts of patients with Hansen's disease.
对100名与麻风病人无血缘关系且无已知麻风病接触史的健康成年巴西男性进行了光田试验。三名瘤型麻风病人作为光田试验阴性对照。所用麻风菌素来自按照世界卫生组织标准制备的感染麻风分枝杆菌的犰狳组织。根据在马德里举行的第六届国际麻风病大会（1953年）的建议，对麻风菌素接种的临床反应进行分级。组织学反应根据作者基于肉芽肿反应强度的标准进行分级。77名临床阳性、19名可疑以及4名临床阴性光田反应者中的1名在组织学上显示出不同强度的阳性反应。当将两种读数结果分为阴性和非阴性两类时，光田反应的临床读数与组织学读数之间显示出显著关联。然而，组织学上阳性的麻风菌素反应的不同程度在临床阳性的光田反应者中随机分布。在与麻风病人无已知接触史的健康受试者中观察到的组织学阴性光田反应者的频率（3%），与有关麻风病人健康接触者的相关文献报道的频率相比显著较低。
