Department of Neuroscience, Uppsala University, Uppsala, Sweden.
St Erik Eye Hospital, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Br J Ophthalmol. 2019 Aug;103(8):1190-1194. doi: 10.1136/bjophthalmol-2017-310416. Epub 2019 Mar 6.
BACKGROUND/AIMS: Pseudoexfoliation syndrome (PEX) is characterised by the production and accumulation of extracellular fibrillar material in the anterior segment of the eye. The pathogenesis of PEX is multifactorial with genetic factors and ageing as contributing factors. Previously, an increased concentration of beta-crystalline B2 (CRYBB2) was observed in the aqueous humour (AH) in eyes with PEX in a pooled material. Here, the protein content was examined on individual basis.
During cataract surgery, AH was sampled from patients with and without PEX, 10 eyes in each group. The proteins were digested and labelled with isotopomeric dimethyl labels, separated with high-pressure liquid chromatography and analysed in an Orbitrap mass analyzer.
The concentration of complement factor 3, kininogen-1, antithrombin III and vitamin D-binding protein was increased in all eyes with PEX. Retinol-binding protein 3, glutathione peroxidase, calsyntenin-1 and carboxypeptidase E were decreased in eyes with PEX. Beta-crystalline B1 and CRYBB2 and gamma-crystalline D were up to eightfold upregulated in 4 of 10 in eyes with PEX. CONCLUSION : The results indicate that oxidative stress and inflammation are contributing factors in the formation of PEX. Knowledge about the proteome in PEX is relevant for understanding this condition.
背景/目的：假性剥脱综合征（PEX）的特征是眼前段细胞外纤维状物质的产生和积累。PEX 的发病机制是多因素的，遗传因素和衰老都是促成因素。先前，在汇集的材料中观察到 PEX 眼中房水中β-晶状体 B2（CRYBB2）的浓度增加。在这里，对个体基础上的蛋白质含量进行了检查。
在白内障手术过程中，从有和没有 PEX 的患者中抽取房水，每组 10 只眼。将蛋白质消化并标记为同位素二甲基标记物，用高压液相色谱分离，并在轨道阱质谱分析仪中分析。
所有 PEX 眼中的补体因子 3、激肽原-1、抗凝血酶 III 和维生素 D 结合蛋白的浓度增加。PEX 眼中的视黄醇结合蛋白 3、谷胱甘肽过氧化物酶、钙结合蛋白-1 和羧肽酶 E 减少。在 4 只 PEX 眼中，β-晶状体 B1 和 CRYBB2 和γ-晶状体 D 上调了 8 倍。
结果表明，氧化应激和炎症是 PEX 形成的促成因素。对 PEX 蛋白质组的了解对于理解这种情况很重要。
