Lo Presti F, Riffard S, Meugnier H, Reyrolle M, Lasne Y, Grimont P A, Grimont F, Benson R F, Brenner D J, Steigerwalt A G, Etienne J, Freney J
Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):1949-1957. doi: 10.1099/00207713-51-6-1949.
Novel Legionella-like isolates, strains Montbéliard A1T and Gréoux 11 D13T, isolated from two different French water sources, were studied taxonomically and phylogenetically. Morphological and biochemical characterization revealed that they were Gram-negative, aerobic, non-spore-forming bacilli with a cut-glass appearance that grew only on L-cysteine-supplemented buffered charcoal yeast extract agar. Phenotypic characterization using fatty acid and ubiquinone profiles and SDS-PAGE analysis confirmed that they were closely related, but distinct from, other species of the genus Legionella, since serotyping could not relate them to any existing serogroup. Genotypic profiles generated by randomly amplified polymorphic DNA and 16S-23S rDNA spacer region PCR analyses were unique for each of these isolates. DNA-DNA relatedness values of strains Montbéliard A1T and Gréoux 11 D13T to each other and to other Legionella type strains were less than 25%. Phylogenetic affiliation of these organisms obtained by 16S rDNA sequence comparisons confirmed that they were distinct from any other known Legionella species. All the above results confirm that these strains constitute two novel species for which the names Legionella gresilensis sp. nov. (type strain Gréoux 11 D13T = ATCC 700509T = CIP 106631T) and Legionella beliardensis sp. nov. (type strain Montbéliard A1T = ATCC 700512T = CIP 106632T) are proposed.
对从法国两个不同水源分离出的新型嗜肺军团菌样菌株——蒙贝利亚尔A1T菌株和格雷乌11 D13T菌株，进行了分类学和系统发育研究。形态学和生化特征表明，它们是革兰氏阴性、需氧、无芽孢杆菌，呈玻璃切割样外观，仅在添加L-半胱氨酸的缓冲活性炭酵母提取物琼脂上生长。利用脂肪酸和泛醌谱以及SDS-PAGE分析进行的表型特征鉴定证实，它们与嗜肺军团菌属的其他物种密切相关，但又有所不同，因为血清分型无法将它们与任何现有的血清群联系起来。通过随机扩增多态性DNA和16S-23S rDNA间隔区PCR分析产生的基因型谱对于这些分离株中的每一个都是独特的。蒙贝利亚尔A1T菌株和格雷乌11 D13T菌株彼此之间以及与其他嗜肺军团菌模式菌株的DNA-DNA相关性值均小于25%。通过16S rDNA序列比较获得的这些生物体的系统发育归属证实，它们与任何其他已知的嗜肺军团菌物种都不同。上述所有结果证实，这些菌株构成了两个新物种，为此提议将其命名为格雷西嗜肺军团菌新种（模式菌株格雷乌11 D13T = ATCC 700509T = CIP 106631T）和贝利阿尔德嗜肺军团菌新种（模式菌株蒙贝利亚尔A1T = ATCC 700512T = CIP 106632T）。
