Institut für Medizinische Mikrobiologie und Hygiene, TU Dresden, 01307 Dresden, Germany.
Institut für Mikrobiologie, TU Dresden, 01062 Dresden, Germany.
Int J Syst Evol Microbiol. 2010 Nov;60(Pt 11):2557-2562. doi: 10.1099/ijs.0.017863-0. Epub 2009 Dec 11.
Legionella-like isolates, strains W03-356(T), W03-357 and W03-359, from three independent water samples from the river Elbe, Germany, were analysed by using a polyphasic approach. Morphological and biochemical characterization revealed that they were Gram-negative, aerobic, non-spore-forming bacilli with a cut glass colony appearance that grew only on L-cysteine-supplemented buffered charcoal yeast extract agar. Phylogenetic analysis based on sequence comparisons of the 16S rRNA, macrophage infectivity potentiator (mip), gyrase subunit A (gyrA), ribosomal polymerase B (rpoB) and RNase P (rnpB) genes confirmed that the three isolates were distinct from recognized species of the genus Legionella. Phenotypic characterization of strain W03-356(T) based on fatty acid profiles confirmed that it was closely related to Legionella rubrilucens ATCC 35304(T) and Legionella pneumophila ATCC 33152(T), but distinct from other species of the genus Legionella. Serotyping of the isolates showed that they were distinct from all recognized species of the genus Legionella. Strains W03-356(T), W03-357 and W03-359 are thus considered to represent a novel species of the genus Legionella, for which the name Legionella dresdenensis sp. nov. is proposed. The type strain is W03-356(T) (=DSM 19488(T)=NCTC 13409(T)).
从德国易北河的三个独立水样中分离出的军团菌样分离株、菌株 W03-356(T)、W03-357 和 W03-359，采用多相分析方法进行了分析。形态学和生化特征分析表明，它们是革兰氏阴性、需氧、非孢子形成的杆菌，具有玻璃切割状菌落外观，仅在含有 L-半胱氨酸的缓冲木炭酵母提取物琼脂上生长。基于 16S rRNA、巨噬细胞感染增强子 (mip)、gyrase 亚基 A (gyrA)、核糖体聚合酶 B (rpoB) 和 RNA 聚合酶 P (rnpB) 基因的序列比较进行的系统发育分析证实，这三个分离株与军团菌属的公认种不同。基于脂肪酸图谱对菌株 W03-356(T)的表型特征进行的表征证实，它与军团菌 rubrilucens ATCC 35304(T)和军团菌 pneumophila ATCC 33152(T)密切相关，但与军团菌属的其他种不同。分离株的血清分型表明，它们与军团菌属的所有公认种均不同。因此，菌株 W03-356(T)、W03-357 和 W03-359 被认为代表军团菌属的一个新种，提议将其命名为 Legionella dresdenensis sp. nov.。模式菌株为 W03-356(T)(=DSM 19488(T)=NCTC 13409(T))。
