Park Mi-Yeoun, Ko Kwan Soo, Lee Hae Kyung, Park Man-Suk, Kook Yoon-Hoh
Laboratory of Rickettsial and Zoonotic Disease, Department of Microbiology, Korean National Institute of Health, Seoul 122-701, Korea.
Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine and Clinical Research Institute, Seoul National University Hospital, Seoul 110-799, Korea.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):77-80. doi: 10.1099/ijs.0.02268-0.
Three Legionella-like micro-organisms, isolated from cooling tower water of a building in Busan, Korea, were characterized by a variety of biochemical and molecular phylogenetic tests. Analyses of whole-cell fatty acids and results of biochemical tests revealed that these three isolates are distinct from previously described Legionella species. Furthermore, results of comparative analyses of 16S rDNA (1476-1488 bp), mip (408 bp) and rpoB (300 bp) sequences also confirmed that these strains represent a novel species within the genus Legionella. The 16S rDNA sequences of the three Korean isolates had similarities of less than 95.8% to other Legionella species. Phylogenetic trees formed by analysis of the 16S rRNA, rpoB and mip genes revealed that the isolates formed a distinct cluster within the genus Legionella. Based on the evaluated phenotypic and phylogenetic characteristics, it is proposed that these Korean isolates from water be classified as a novel species, Legionella busanensis sp. nov.; the type strain is strain K9951T (=KCTC 12084T =ATCC BAA-518T).
从韩国釜山一栋建筑的冷却塔水中分离出的三种类军团菌微生物，通过各种生化和分子系统发育测试进行了表征。全细胞脂肪酸分析和生化测试结果表明，这三种分离株与先前描述的军团菌物种不同。此外，16S rDNA（1476 - 1488 bp）、mip（408 bp）和rpoB（300 bp）序列的比较分析结果也证实，这些菌株代表军团菌属内的一个新物种。这三株韩国分离株的16S rDNA序列与其他军团菌物种的相似性低于95.8%。通过对16S rRNA、rpoB和mip基因分析形成的系统发育树表明，这些分离株在军团菌属内形成了一个独特的簇。基于评估的表型和系统发育特征，建议将这些来自水的韩国分离株归类为一个新物种，釜山军团菌（Legionella busanensis sp. nov.）；模式菌株为K9951T（=KCTC 12084T =ATCC BAA - 518T）。
