Chassoux D, Salomon J C
Int J Cancer. 1975 Oct 15;16(4):515-25. doi: 10.1002/ijc.2910160402.
The experimental treatment of a rat sarcoma (McFiFi 2) by intratumoral injection of BCG2 is described. Tumors which have a mean diameter of less than 10 mm at the beginning of treatment are fully susceptible to BCG, although spontaneous regression is not observed at this stage. The effective dose of living BCG ranges from two injections of 0.1 mg to two injections of 1 mg, given IT at an interval of 7 days. The permanent cure of a proporation of the tumors may also be induced by IT injection of a similar dose of heat-killed BCG or of MER, or of 10(9) heat-killed C. parvum, according to the same schedule. Preimmunization of the rats with living BCG does not improve the efficiency of heat-killed BCG. Direct contact between the therapeutic material and the tumor cells is critical. If rats are grafted with two pieces of the same tumor in widely separated sites, the intratumoral treatment of only one of these tumors with living BCG is sufficient to induce regression of both tumors in 50% of the animals. The effect of BCG is counteracted by injection of silica or by ingestion of polaramine. The same intratumoral treatment with living BCG was applied to different rat and mouse tumors. Only McFiFi 2 tumors were cured by intralesional BCG. C3H mouse plasmocytoma 5563 was not cured by intratumoral BCG but its growth could be prevented by mixing BCG and tumor cells at the time of grafting; this tumor was considered to be of medium susceptibility. However, until there is definite proof that the two mechanisms are identical, one should consider the regression and cure of a growing tumor, and the prevention of tumor growth, as two different phenomena. The clinical treatment of human tumors resembles the first experimental procedure more closely than the second.
本文描述了通过瘤内注射卡介苗（BCG2）对大鼠肉瘤（McFiFi 2）进行的实验性治疗。治疗开始时平均直径小于10毫米的肿瘤对卡介苗完全敏感，尽管在此阶段未观察到自发消退。活卡介苗的有效剂量范围为两次注射0.1毫克至两次注射1毫克，瘤内注射，间隔7天。按照相同的方案，瘤内注射相似剂量的热灭活卡介苗、MER或10(9)热灭活微小隐孢子虫也可诱导一定比例的肿瘤永久治愈。用活卡介苗对大鼠进行预免疫并不能提高热灭活卡介苗的疗效。治疗物质与肿瘤细胞之间的直接接触至关重要。如果在大鼠的广泛分离部位移植两块相同的肿瘤，仅对其中一个肿瘤进行瘤内注射活卡介苗就足以使50%的动物体内的两个肿瘤都发生消退。注射二氧化硅或摄入扑尔敏可抵消卡介苗的作用。对不同的大鼠和小鼠肿瘤采用相同的瘤内注射活卡介苗治疗方法。只有McFiFi 2肿瘤可通过瘤内注射卡介苗治愈。C3H小鼠浆细胞瘤5563不能通过瘤内注射卡介苗治愈，但在接种时将卡介苗与肿瘤细胞混合可阻止其生长；该肿瘤被认为具有中等敏感性。然而，在有确凿证据证明这两种机制相同时，应将正在生长的肿瘤的消退和治愈以及肿瘤生长的预防视为两种不同的现象。人类肿瘤的临床治疗与第一种实验方法的相似性比与第二种实验方法的相似性更高。
