Ferluga J, Schorlemmer H U, Baptista L C, Allison A C
Clin Exp Immunol. 1978 Mar;31(3):512-7.
Mouse peritoneal macrophages were activated in serum-free culture with lipopolysaccharide, dextran sulphate or C3b. They were found to liberate into the culture medium a cytolytic factor and a factor which was pharmacologically indistinguishable from C3a. The cytolytic activity was neutralized by antiserum against C3a. These results suggest that macrophages can be activated by C3b (or agents that generate C3b) to cleave C3, which they themselves produce. In this way the activated macrophages form C3a, which appears to be a major factor in macrophage-mediated cytolysis. These findings have implications in tumour immunity and in the pathogenesis of inflammatory reactions.
小鼠腹腔巨噬细胞在无血清培养中用脂多糖、硫酸葡聚糖或C3b激活。发现它们将一种细胞溶解因子和一种在药理学上与C3a无法区分的因子释放到培养基中。细胞溶解活性被抗C3a抗血清中和。这些结果表明巨噬细胞可被C3b（或产生C3b的试剂）激活以裂解它们自身产生的C3。通过这种方式，活化的巨噬细胞形成C3a，这似乎是巨噬细胞介导的细胞溶解的主要因素。这些发现对肿瘤免疫和炎症反应的发病机制具有启示意义。
