[瘢痕成纤维细胞中Bcl-2和Fas基因表达的实验研究]

[Experimental study of Bcl-2 and Fas gene expression in fibroblast of scar].

作者信息

Liu Y, Ren L S, Cen Y

机构信息

Department of Burn and Plastic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu Sichuan, P. R. China 610041.

出版信息

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2001 Nov;15(6):351-3.

PMID:11762221

Abstract

OBJECTIVE

To explore the effect of Fas/Apo-1 and Bcl-2 gene expression on mechanism of scar formation.

METHODS

Immunohistochemical method was applied to defect the expression of Fas and Bcl-2 protein in fibroblasts from 10 cases with normal skin, 10 cases with hypertrophic scar and 10 cases with keloid.

RESULTS

The positive expression rate of Bcl-2 protein in keloid was 83.2%, significantly higher than that in hypertrophic scar (38.6%), (P < 0.01), and the positive expression rate in hypertrophic scar and keloid was higher than that in normal skin (6.78%), (P < 0.01). But the positive expression rate of Fas/Apo-1 protein was 78.4% in normal skin 80.4% in hypertrophic scar, 84.4% in keloid respectively, which showed no significant difference among them (P > 0.05).

CONCLUSION

Bcl-2 gene but Fas gene may take part in the formation of pathologic scar.

摘要

目的

探讨Fas/Apo-1和Bcl-2基因表达在瘢痕形成机制中的作用。

方法

采用免疫组织化学方法检测10例正常皮肤、10例增生性瘢痕及10例瘢痕疙瘩成纤维细胞中Fas和Bcl-2蛋白的表达。

结果

瘢痕疙瘩中Bcl-2蛋白阳性表达率为83.2%，明显高于增生性瘢痕（38.6%），（P<0.01），增生性瘢痕和瘢痕疙瘩中Bcl-2蛋白阳性表达率高于正常皮肤（6.78%），（P<0.01）。但Fas/Apo-1蛋白阳性表达率在正常皮肤为78.4%，增生性瘢痕为80.4%，瘢痕疙瘩为84.4%，三者之间差异无统计学意义（P>0.05）。