Ohshima M, Noguchi Y, Ito M, Maeno M, Otsuka K
Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan.
J Periodontal Res. 2001 Dec;36(6):377-83. doi: 10.1034/j.1600-0765.2001.360605.x.
To ascertain whether periodontal fibroblasts could be involved in the pathogenesis of periodontal pocket formation, the chemotactic activity of periodontal ligament fibroblast-conditioned medium (PLF-CM) and gingival fibroblast-conditioned medium (GF-CM) for gingival epithelial cells was examined using a modified Boyden chamber assay. Both PLF-CM and GF-CM possessed significant chemotactic activity, which was decreased markedly by treatment with anti-human hepatocyte growth factor (HGF) neutralizing antibody. Furthermore, the chemotactic activity of PLF-CM and GF-CM was well correlated with HGF content. These results show that PLF and GF secrete an HGF-like factor, and suggest that such a factor derived from periodontal fibroblasts might play a role in epithelial apical migration in periodontitis.
为确定牙周成纤维细胞是否参与牙周袋形成的发病机制，采用改良的Boyden小室分析法检测了牙周膜成纤维细胞条件培养基（PLF-CM）和牙龈成纤维细胞条件培养基（GF-CM）对牙龈上皮细胞的趋化活性。PLF-CM和GF-CM均具有显著的趋化活性，用抗人肝细胞生长因子（HGF）中和抗体处理后，趋化活性明显降低。此外，PLF-CM和GF-CM的趋化活性与HGF含量密切相关。这些结果表明，PLF和GF分泌一种HGF样因子，提示牙周成纤维细胞来源的这种因子可能在牙周炎上皮向根尖迁移中起作用。
