Sutter V L, Kwok Y Y, Finegold S M
Appl Microbiol. 1972 Feb;23(2):268-75. doi: 10.1128/am.23.2.268-275.1972.
A modified Bauer-Kirby-Sherris-Turck method for disc susceptibility testing of anaerobic bacteria is presented. When tetracycline was used against 100 strains of Bacteroides fragilis as a model, reasonably reproducible results were obtained after overnight incubation in both the GasPak atmosphere and an atmosphere achieved by adding 10% CO(2) to a mixture of 10% H(2) and 90% N(2). The minimal inhibitory concentration for the strains determined by the agar dilution technique correlated well with the results of disc tests performed in the GasPak atmosphere with 30-mug tetracycline discs. Among 63 strains isolated from 1970 to the present, only 24 (38.1%) were found to be susceptible to tetracycline.
本文介绍了一种用于厌氧菌纸片药敏试验的改良鲍尔-柯比-谢里斯-图尔克方法。以四环素针对100株脆弱拟杆菌作为模型，在GasPak环境以及通过向10%氢气和90%氮气的混合气体中添加10%二氧化碳所营造的环境中过夜培养后，均获得了相当可重复的结果。通过琼脂稀释技术测定的菌株最低抑菌浓度与在GasPak环境中使用30μg四环素纸片进行的纸片试验结果高度相关。在1970年至今分离出的63株菌株中，仅24株（38.1%）被发现对四环素敏感。
