Zhang J, Wang Z, Li M, Wang B
School of Life Sciences, Zhongshan University, Guangzhou 510275.
Ying Yong Sheng Tai Xue Bao. 2000 Aug;11(4):631-6.
This review surveys kinds of markers such as allozyme, AFLP, RAPD, SSR, RFLP and their application in molecular ecology of plant populations. The advantages and disadvantages of these molecular markers in plant population research are discussed based on the comparison of the methods and the experience in research work. It is considered emphatically that the adoption of the molecular markers is dependent on the various research purposes and different quality and quantity of the molecular information are acquired from various molecular marker methods.
本文综述了等位酶、扩增片段长度多态性(AFLP)、随机扩增多态性DNA(RAPD)、简单序列重复(SSR)、限制性片段长度多态性(RFLP)等各类分子标记及其在植物种群分子生态学中的应用。基于方法比较和研究工作经验,讨论了这些分子标记在植物种群研究中的优缺点。着重指出,分子标记的选用取决于各种研究目的,并且从不同的分子标记方法可获得质量和数量各异的分子信息。