用于烟曲霉分离株指纹图谱分析的限制性片段长度多态性、微卫星长度多态性及随机扩增多态性DNA分析的比较
Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates.
作者信息
Bart-Delabesse E, Sarfati J, Debeaupuis J P, van Leeuwen W, van Belkum A, Bretagne S, Latge J P
机构信息
Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor, Créteil, France.
出版信息
J Clin Microbiol. 2001 Jul;39(7):2683-6. doi: 10.1128/JCM.39.7.2683-2686.2001.
Abstract
Aspergillus fumigatus fingerprints generated by random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) upon hybridization with repeated DNA sequences, and PCR detection of microsatellite length polymorphism (MLP) were compared among 67 isolates. In contrast to RAPD, RFLP and MLP gave discriminating and significantly concordant genotyping results.
摘要
在67株烟曲霉菌株中，比较了通过随机扩增多态性DNA（RAPD）、与重复DNA序列杂交后的限制性片段长度多态性（RFLP）以及微卫星长度多态性（MLP）的PCR检测所产生的指纹图谱。与RAPD不同，RFLP和MLP给出了具有区分性且显著一致的基因分型结果。
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