He P, Warren R F, Zhao T, Shan L, Zhu L, Tang X, Zhou J M
Department of Plant Pathology, Kansas State University, Manhattan 66506, USA.
Mol Plant Microbe Interact. 2001 Dec;14(12):1453-7. doi: 10.1094/MPMI.2001.14.12.1453.
The tomato Pti5 gene encodes a pathogen-inducible ethylene response element-binding protein-like transcription factor that interacts with the disease resistance gene product Pto. Overexpression of Pti5 or Pti5-VP16, a translational fusion with a constitutive transcriptional activation domain, in tomato enhanced resistance to Pseudomonas syringae pv. tomato. Constitutive expression of Pti5 or Pti5-VP16 did not affect the basal level of pathogenesis-related gene expression, but it accelerated pathogen-induced expression of GluB and Catalase. The results demonstrate a positive role of Pti5 in defense gene regulation and disease resistance and suggest that a pathogen-activated posttranscriptional regulatory step is necessary for the pathogen induction of the defense gene expression.
番茄Pti5基因编码一种病原体诱导型乙烯反应元件结合蛋白样转录因子，该因子与抗病基因产物Pto相互作用。在番茄中过表达Pti5或Pti5-VP16（一种与组成型转录激活域的翻译融合体）可增强对丁香假单胞菌番茄致病变种的抗性。Pti5或Pti5-VP16的组成型表达不影响病程相关基因表达的基础水平，但加速了病原体诱导的GluB和过氧化氢酶的表达。结果表明Pti5在防御基因调控和抗病性中发挥积极作用，并表明病原体激活的转录后调控步骤对于病原体诱导防御基因表达是必要的。
