Sirois P, Moore E G, Orange R P
Agents Actions. 1979 Oct;9(4):337-43. doi: 10.1007/BF01970658.
Arachidonic acid stimulates the release of SRS-A from the peritoneal cavity of sensitized rats or from rat peritoneal cells incubated in vitro. When rat peritoneal cells are incubated in the presence of tritiated arachidonic acid, significant amounts of radioactivity migrate in parallel to bioactivity on purification with Amberlite XAD-8, DE-52, Silicic acid and Sephadex LH-20. Lipoxidase (E.C. 1.13.1.13 and E.C. 1.13.11.12) inactivates mouse, rat and human SRS-A in a concentration-dependent pattern. Following extensive purification, rat SRS-A is also inactivated by the 2 x crystalline suspension of lipoxidase. These findings suggest (a) that SRS-A is a metabolite of arachidonic acid and (b) because of the strict specificity of lipoxidase, that the SRS-A molecule contains a cis, cis-1, 4-pentadiene and a structure very close either to arachidonic acid, to linoleic acid or to linolenic acid.
花生四烯酸可刺激致敏大鼠腹腔或体外培养的大鼠腹腔细胞释放慢反应物质-A（SRS-A）。当用氚标记的花生四烯酸孵育大鼠腹腔细胞时，在用Amberlite XAD - 8、DE - 52、硅酸和葡聚糖凝胶LH - 20进行纯化时，大量放射性物质与生物活性平行迁移。脂氧化酶（E.C. 1.13.1.13和E.C. 1.13.11.12）以浓度依赖的方式使小鼠、大鼠和人类的SRS - A失活。经过广泛纯化后，大鼠SRS - A也会被脂氧化酶的2倍结晶悬浮液失活。这些发现表明：（a）SRS - A是花生四烯酸的代谢产物；（b）由于脂氧化酶的严格特异性，SRS - A分子含有顺，顺-1,4 - 戊二烯，并且其结构与花生四烯酸、亚油酸或亚麻酸非常接近。
