Edrington T S, Harvey R B, Farrington L A, Nisbet D J
Southern Plains Agricultural Research Service, US Department of Agriculture, College Station, Texas 77845, USA.
J Food Prot. 2001 Dec;64(12):2067-70. doi: 10.4315/0362-028x-64.12.2067.
The antibiotics apramycin and carbadox were fed to growing swine, and the prevalence of Salmonella isolates that are resistant to apramycin and related aminoglycoside antibiotics was examined. Three hundred twelve Salmonella-positive pigs raised on one of five farms in an integrated swine operation and slaughtered at a central plant were used. All farms fed carbadox during the grower phase, and two farms administered apramycin during the first 21 days of age. Ileocolic lymph nodes and cecal contents were sampled at slaughter. One hundred of the 312 pigs were randomly selected to examine apramycin- and carbadox-resistant Salmonella infection, while all 312 pigs were used to evaluate the association between apramycin exposure and infection with Salmonella organisms resistant to amikacin, gentamicin, kanamycin, and streptomycin. Antimicrobial resistance was determined using disk diffusion and breakpoint concentrations. Apramycin treatment appeared to have little effect on apramycin- (12.5 versus 20.9%) or streptomycin- (76.4 versus 73.5%) resistant Salmonella isolates when averaged across farms and compared to control animals. Feeding carbadox resulted in carbadox-resistant Salmonella infection in only 5.3% of the isolates on one farm. The prevalence of amikacin-, gentamicin-, and kanamycin-resistant Salmonella isolates on farms feeding apramycin and carbadox were 0, 0, and 1.8%, respectively. Serogroup B was the most prevalent serogroup isolated, followed by C1 and E1. Apramycin and carbadox treatment did not appear to have any effect on the serogroup isolated. Subtherapeutic use of carbadox and apramycin did not appear to increase the prevalence of antimicrobial-resistant Salmonella in market-age swine.
将阿普拉霉素和卡巴多司喂给生长中的猪，并检测对阿普拉霉素和相关氨基糖苷类抗生素耐药的沙门氏菌分离株的流行情况。在一个一体化养猪场的五个农场之一饲养并在中央屠宰场屠宰的312头沙门氏菌阳性猪被用于研究。所有农场在育肥阶段都饲喂卡巴多司，两个农场在猪21日龄前给予阿普拉霉素。屠宰时采集回盲部淋巴结和盲肠内容物样本。312头猪中随机选取100头检查对阿普拉霉素和卡巴多司耐药的沙门氏菌感染情况，而所有312头猪都用于评估阿普拉霉素暴露与对阿米卡星、庆大霉素、卡那霉素和链霉素耐药的沙门氏菌感染之间的关联。使用纸片扩散法和断点浓度测定抗菌药物耐药性。将各农场的数据平均后与对照动物相比，阿普拉霉素处理似乎对阿普拉霉素耐药（12.5%对20.9%）或链霉素耐药（76.4%对73.5%）的沙门氏菌分离株影响不大。在一个农场，饲喂卡巴多司仅导致5.3%的分离株出现对卡巴多司耐药的沙门氏菌感染。在饲喂阿普拉霉素和卡巴多司的农场中，对阿米卡星、庆大霉素和卡那霉素耐药的沙门氏菌分离株的流行率分别为0、0和1.8%。分离出的最常见血清型是B型，其次是C1型和E1型。阿普拉霉素和卡巴多司处理似乎对分离出的血清型没有任何影响。卡巴多司和阿普拉霉素的亚治疗剂量使用似乎并未增加上市猪中耐抗菌药物沙门氏菌的流行率。
