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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Storer A C, Cornish-Bowden A

Biochem J. 1976 Oct 1;159(1):1-5. doi: 10.1042/bj1590001.

A simple method is described for calculating the free concentrations of all species in a mixture of several ionic components that associate at equilibrium to any extent and with any stoicheiometry. 2. It can readily be adapted to take account of species such as protons for which the free rather than the total concentrations are controlled. 3. It was applied to mixtures of adenine nucleotides, Mg2+ and other ions relevant to the study of glucokinase (EC 2.7.1.2), but the qualitative conclusions are not peculiar to this system. 4. ATP exists in a high and nearly constant proportion (about 80%) as MgATP2- in solutions in which the total MgCl2 concentration exceeds the total ATP concentration by 1-10 mM. 5. By contrast, the proportion of ATP present as MgATP2- varies greatly if the total MgCl2 and total ATP concentrations are varied in constant proportion.

描述了一种简单方法，用于计算几种离子成分混合物中所有物种的游离浓度，这些离子成分在平衡时会以任何程度和任何化学计量比发生缔合。2. 它可以很容易地进行调整，以考虑诸如质子等物种，其游离浓度而非总浓度受到控制。3. 该方法应用于腺嘌呤核苷酸、Mg2+和其他与葡萄糖激酶（EC 2.7.1.2）研究相关的离子混合物，但定性结论并非该系统所特有。4. 在总MgCl2浓度比总ATP浓度高1 - 10 mM的溶液中，ATP以MgATP2-的形式存在的比例很高且几乎恒定（约80%）。5. 相比之下，如果总MgCl2和总ATP浓度以恒定比例变化，以MgATP2-形式存在的ATP比例会有很大变化。

Storer A C, Cornish-Bowden A

Biochem J. 1976 Oct 1;159(1):1-5. doi: 10.1042/bj1590001.

A simple method is described for calculating the free concentrations of all species in a mixture of several ionic components that associate at equilibrium to any extent and with any stoicheiometry. 2. It can readily be adapted to take account of species such as protons for which the free rather than the total concentrations are controlled. 3. It was applied to mixtures of adenine nucleotides, Mg2+ and other ions relevant to the study of glucokinase (EC 2.7.1.2), but the qualitative conclusions are not peculiar to this system. 4. ATP exists in a high and nearly constant proportion (about 80%) as MgATP2- in solutions in which the total MgCl2 concentration exceeds the total ATP concentration by 1-10 mM. 5. By contrast, the proportion of ATP present as MgATP2- varies greatly if the total MgCl2 and total ATP concentrations are varied in constant proportion.

描述了一种简单方法，用于计算几种离子成分混合物中所有物种的游离浓度，这些离子成分在平衡时会以任何程度和任何化学计量比发生缔合。2. 它可以很容易地进行调整，以考虑诸如质子等物种，其游离浓度而非总浓度受到控制。3. 该方法应用于腺嘌呤核苷酸、Mg2+和其他与葡萄糖激酶（EC 2.7.1.2）研究相关的离子混合物，但定性结论并非该系统所特有。4. 在总MgCl2浓度比总ATP浓度高1 - 10 mM的溶液中，ATP以MgATP2-的形式存在的比例很高且几乎恒定（约80%）。5. 相比之下，如果总MgCl2和总ATP浓度以恒定比例变化，以MgATP2-形式存在的ATP比例会有很大变化。