Wang Jen-Ren, Tuan Yen-Chang, Tsai Huey-Pin, Yan Jing-Jou, Liu Ching-Chuan, Su Ih-Jen
Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
J Clin Microbiol. 2002 Jan;40(1):10-5. doi: 10.1128/JCM.40.1.10-15.2002.
Two outbreaks of hand-foot-and-mouth disease (HFMD) occurred in Taiwan between 1998 and 2000. Enteroviruses were isolated from a total of 1,892 patients in this laboratory during this period. Of the virus isolates, enterovirus 71 (EV71) was diagnosed in 44.4% of the patients (132 of 297) in 1998, 2% (13 of 646) in 1999, and 20.5% (195 of 949) in 2000. Genetic analyses of the 5'-untranslated and VP1 regions of EV71 isolates by reverse transcription-PCR and sequencing were performed to understand the diversity of EV71 in these outbreaks of HFMD. Most EV71 isolates from the 1998 epidemic belonged to genotype C, while only one-tenth of the isolates were genotype B. Interestingly, all EV71 isolates tested from 1999 to 2000 belonged to genotype B. This study indicated that two genogroups of EV71 capable of inducing severe clinical illness have been circulating in Taiwan. Furthermore, the predominant EV71 genotypes responsible for each of the two major HFMD outbreaks within the 3-year period in Taiwan were different.
1998年至2000年期间，台湾发生了两起手足口病疫情。在此期间，本实验室从总共1892名患者中分离出肠道病毒。在分离出的病毒中，1998年44.4%的患者（297例中的132例）被诊断为肠道病毒71型（EV71），1999年为2%（646例中的13例），2000年为20.5%（949例中的195例）。通过逆转录聚合酶链反应和测序对EV71分离株的5'非翻译区和VP1区进行基因分析，以了解这些手足口病疫情中EV71的多样性。1998年疫情中的大多数EV71分离株属于C基因型，而只有十分之一的分离株是B基因型。有趣的是，1999年至2000年检测的所有EV71分离株都属于B基因型。本研究表明，两种能够引起严重临床疾病的EV71基因群一直在台湾流行。此外，在台湾3年期间的两起主要手足口病疫情中，各自主要的EV71基因型有所不同。
