Weng C, Yu K, Anderson T R, Poysa V
Agriculture and Agri-food Canada, Greenhouse and Processing Crop Research Centre, 2585 Highway 20 E., Harrow, Ontario, N0R 1G0 Canada.
J Hered. 2001 Sep-Oct;92(5):442-6. doi: 10.1093/jhered/92.5.442.
A linkage map was constructed for two Phytophthora sojae Kauf. +Gerd. root rot resistance genes, Rps1a and Rps7, in soybean (Glycine max (L.) Merr.) using microsatellite or simple sequence repeat (SSR) markers. An F2 population consisting of 81 individuals derived from a cross between OX281, which carries Rps7, and Mukden, which carries Rps1a, was used as the mapping population. A linkage map consisting of 10 SSR markers was first constructed using the computer software MapMaker/EXP 3.0. Rps1a and Rps7 were then placed at two different loci in the same linkage group with LOD scores of 2.88 and 9.16, respectively. Rps1a and Rps7 were linked at a distance of 13.8 cM. Rps1a was flanked by Satt159 (0.7 cM) and Satt009 (3.2 cM). Rps7 was flanked by Satt009 (10.6 cM) and Satt125 (29.1 cM).
利用微卫星或简单序列重复（SSR）标记，构建了大豆（Glycine max (L.) Merr.）中两个大豆疫霉根腐病抗性基因Rps1a和Rps7的连锁图谱。以由携带Rps7的OX281和携带Rps1a的Mukden杂交产生的81个个体组成的F2群体作为作图群体。首先使用计算机软件MapMaker/EXP 3.0构建了一个由10个SSR标记组成的连锁图谱。然后，Rps1a和Rps7被定位在同一连锁群的两个不同位点，LOD值分别为2.88和9.16。Rps1a和Rps7相距13.8 cM。Rps1a两侧分别是Satt159（0.7 cM）和Satt009（3.2 cM）。Rps7两侧分别是Satt009（10.6 cM）和Satt125（29.1 cM）。
