MOA Key Lab of Soybean Biology (Beijing), the National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, People's Republic of China.
Theor Appl Genet. 2013 Jun;126(6):1555-61. doi: 10.1007/s00122-013-2073-1. Epub 2013 Mar 7.
Phytophthora root rot (PRR), caused by Phytophthora sojae Kaufmann & Gerdemann, is one of the most destructive diseases of soybean [Glycine max (L.) Merr.]. Deployment of resistance genes is the most economical and effective way of controlling the disease. The soybean cultivar 'Yudou 29' is resistant to many P. sojae isolates in China. The genetic basis of the resistance in 'Yudou 29' was elucidated through an inheritance study and molecular mapping. In response to 25 P. sojae isolates, 'Yudou 29' displayed a new resistance reaction pattern distinct from those of differentials carrying known Rps genes. A population of 214 F2:3 families from a cross between 'Jikedou 2' (PRR susceptible) and 'Yudou 29' was used for Rps gene mapping. The segregation fit a ratio of 1:2:1 for resistance:segregation:susceptibility within this population, indicating that resistance in 'Yudou 29' is controlled by a single dominant gene. This gene was temporarily named RpsYD29 and mapped on soybean chromosome 03 (molecular linkage group N; MLG N) flanked by SSR markers SattWM82-50 and Satt1k4b at a genetic distance of 0.5 and 0.2 cM, respectively. Two nucleotide binding site-leucine rich repeat (NBS-LRR) type genes were detected in the 204.8 kb region between SattWM82-50 and Satt1k4b. These two genes showed high similarity to Rps1k in amino acid sequence and could be candidate genes for PRR resistance. Based on the phenotype reactions and the physical position on soybean chromosome 03, RpsYD29 might be a novel allele at, or a novel gene tightly linked to, the Rps1 locus.
大豆疫霉根腐病（PRR）是由大豆疫霉菌（Phytophthora sojae Kaufmann & Gerdemann）引起的，是大豆最具破坏性的疾病之一。利用抗病基因是防治该病害最经济有效的方法。大豆品种‘育豆 29’在中国对许多 P. sojae 分离株具有抗性。通过遗传研究和分子图谱对‘育豆 29’的抗性遗传基础进行了阐明。针对 25 个 P. sojae 分离株，‘育豆 29’表现出与携带已知 Rps 基因的对照不同的新的抗性反应模式。用‘吉科豆 2 号’（易感 PRR）与‘育豆 29’杂交产生的 214 个 F2:3 家系群体，用于 Rps 基因作图。该群体的分离比为 1:2:1，即抗性：分离：易感，表明‘育豆 29’的抗性由单个显性基因控制。该基因暂时命名为 RpsYD29，位于大豆第 03 号染色体（分子连锁群 N；MLG N）上，SSR 标记 SattWM82-50 和 Satt1k4b 侧翼，遗传距离分别为 0.5 和 0.2 cM。在 SattWM82-50 和 Satt1k4b 之间的 204.8 kb 区域检测到两个核苷酸结合位点-亮氨酸丰富重复（NBS-LRR）型基因。这两个基因在氨基酸序列上与 Rps1k 高度相似，可能是 PRR 抗性的候选基因。基于表型反应和在大豆第 03 号染色体上的物理位置，RpsYD29 可能是 Rps1 基因座上的一个新等位基因，或与 Rps1 紧密连锁的新基因。
